Structure of PDB 6uwi Chain E

Receptor sequence

>6uwiE (length=646) Species: 1496 (Clostridioides difficile)

EDLDTDNDNIPDSYERNGYTIKDLIAVKWEDSFAEQGYKKYVSNYLESNT
AGDPYTDYEKASGSFDKAIKTEARDPLVAAYPIVGVGMEKLIISTNEHAS
TDQGKTVSRATTNSKTESNTAGVSVNVGNVTTNYSHTTDNSTAVQDSNGE
SWNTGLSINKGESAYINANVRYYNTGTAPMYKVTPTTNLVLDGDTLSTIK
AQENQIGNNLSPGDTYPKKGLSPLALNTMDQFSSRLIPINYDQLKKKLET
TQVSGNFGTKNSSGQIVTEGNSWSDYISQIDSISASIILDTENESYERRV
TAKNLQDPEDKTPELTIGEAIEKAFGATKKDGLLYFNDIPIDESCVELIF
DDNTANKIKDSLKTLSDKKIYNVKLERGMNILIKTPTYFTNFDDYNNYPS
TWSNVNTTNQDGLQGSANKLNGETKIKIPMSELKPYKRYVFSGYSKDPLT
SNSIIVKIKAKEEKTDYLVPEQGYTKFSYEFETTEKDSSNIEITLIGSGT
TYLDNLSITELNSTPEILDEPEVKIPTDQEIMDAHKIYFADLNFNPSTGN
TYINGMYFAPTQTNKEALDYIQKYRVEATLQYSGFKDIGTKDKEMRNYLG
DPNQPKTNYVNLRSYFTGGENIMTYKKLRIYAITPDDRELLVLSVD

3D structure

• PDB: 6uwi Structure of the cell-binding component of theClostridium difficilebinary toxin reveals a di-heptamer macromolecular assembly.
• Chain: E
• Resolution: 3.7 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CA	E	D222 D224 E231 N260 E263 D273	D6 D8 E15 N44 E47 D57
BS02	CA	E	D220 D222 D224 I226 E231	D4 D6 D8 I10 E15
BS03	CA	E	N621 D623 Q644 D734	N391 D393 Q414 D504
BS04	CA	E	D222 D224 E231 N260 E263 D273	D6 D8 E15 N44 E47 D57
BS05	CA	E	D220 D222 D224 I226 E231	D4 D6 D8 I10 E15
BS06	CA	E	N621 D623 Q644 D734	N391 D393 Q414 D504

Gene Ontology

Biological Process

• GO:0051260 protein homooligomerization

Cellular Component

• GO:0005576 extracellular region

External links

• PDB: RCSB:6uwi, PDBe:6uwi, PDBj:6uwi
• PDBsum: 6uwi
• PubMed: 31896582
• UniProt: O32739