Structure of PDB 6u0s Chain E

Receptor sequence

>6u0sE (length=579)

APDIRVPVLIVGGGPAGLTAALALSRYGVPHLLVNRHHGTAHTPRAHLLN
QRTGEIFRDLGIADRVEAHATPGHLMANHVFMSTFAGPEVARIGAYGNGP
DRIGEYRAASPSGLCNLPQHLLEPLLVEAVQEACVGQLRFGHEFVSLEQD
EHGVTSRITDRRTGRDYTVRSDYLIGADGARSRVLAQLGIALDGATGIAR
AVTTWFEADLSRYSAHRPALLYMGAVPGSPPADGRVFVSLRPWTEWLHLT
FPPPTADVDVEDHEAVRAGIRESIGDPTVDVTIKNVSAWEVNSAVAPRYA
SGRVFCVGDAVHQNPPTNGLGLNSAVADSFNLCWKLKLALEGLAGPGLLD
TYHDERQPVGRQIVDRAFRSMVDLIGIPQALGFTEGQSPEEQWRLLDTLH
EDTEEARQRRAALAAATAAIHGQANAHGVELGYRYRTGALVPDGTPEPAD
ERDPELYYRATTWPGARLPHAWLENGRHRCSTLDVTGRGRFTLLTGPGGE
PWRDAARDAALDTGVEVAVLPIGAGGGPRDPYGTWAELREVEESGAVLVR
PDGHVAWRARDHGHAKELPEVMARVLHQP

3D structure

• PDB: 6u0s Structural analyses of the Group A flavin-dependent monooxygenase PieE reveal a sliding FAD cofactor conformation bridging OUT and IN conformations.
• Chain: E
• Resolution: 2.52 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	E	V18 G19 G21 P22 A23 N42 R43 H44 R52 A53 H54 L55 Q126 F151 A184 D185 G186 W296 G315 D316 P323 G326 G328 L329 N330	V11 G12 G14 P15 A16 N35 R36 H37 R45 A46 H47 L48 Q119 F144 A177 D178 G179 W289 G308 D309 P316 G319 G321 L322 N323
BS02	PKS	E	H54 L228 P238 A239 F258 P323 T324 G326 M378	H47 L221 P231 A232 F251 P316 T317 G319 M371

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0004497 monooxygenase activity
• GO:0016491 oxidoreductase activity
• GO:0016709 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
• GO:0071949 FAD binding

External links

• PDB: RCSB:6u0s, PDBe:6u0s, PDBj:6u0s
• PDBsum: 6u0s
• PubMed: 32111738
• UniProt: W0C4C9