Structure of PDB 6sbk Chain E

Receptor sequence

>6sbkE (length=316) Species: 1427 (Bacillus thermoproteolyticus)

ITGTSTVGVGRGVLGDQKNINTTYSTYYYLQDNTRGNGIFTYDAKYRTTL
PGSLWADADNQFFASYDAPAVDAHYYAGVTYDYYKNVHNRLSYDGNNAAI
RSSVHYSQGYNNAFWNGSQMVYGDGDGQTFIPLSGGIDVVAHELTHAVTD
YTAGLIYQNESGAINEAISDIFGTLVEFYANKNPDWEIGEDVYTPGISGD
SLRSMSDPAKYGDPDHYSKRYTGTQDNGGVHINSGIINKAAYLISQGGTH
YGVSVVGIGRDKLGKIFYRALTQYLTPTSNFSQLRAAAVQSATDLYGSTS
QEVASVKQAFDAVGVK

3D structure

• PDB: 6sbk THERMOLYSIN IN COMPLEX WITH FRAGMENT J13
• Chain: E
• Resolution: 1.48 Å

Enzymatic activity

• Enzyme Commision number: 3.4.24.27: thermolysin.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	E	H142 H146 E166	H142 H146 E166
BS02	CA	E	E177 N183 D185 E190	E177 N183 D185 E190
BS03	CA	E	Y193 T194 I197 D200	Y193 T194 I197 D200
BS04	CA	E	D138 E177 D185 E187 E190	D138 E177 D185 E187 E190
BS05	CA	E	D57 D59 Q61	D57 D59 Q61
BS06	47J	E	N159 T222	N159 T222
BS07	L4Z	E	N112 A113 F114 H142 E143 H146 Y157 E166	N112 A113 F114 H142 E143 H146 Y157 E166

Gene Ontology

Molecular Function

• GO:0004222 metalloendopeptidase activity

Biological Process

• GO:0006508 proteolysis

External links

• PDB: RCSB:6sbk, PDBe:6sbk, PDBj:6sbk
• PDBsum: 6sbk
• UniProt: P00800|THER_BACTH Thermolysin (Gene Name=npr)