Structure of PDB 6s6s Chain E

Receptor sequence

>6s6sE (length=470) Species: 192 (Azospirillum brasilense)

MLGFVHTAQRMPDKRPAAERRQDFAEIYARFSDERANEQANRCSQCGVPF
CQVHCPVSNNIPDWLKLTSEGRLEEAYEVSQATNNFPEICGRICPQDRLC
EGNCVIEQSTHGAVTIGSVEKYINDTAWDQGWVKPRTPSRELGLSVGVIG
AGPAGLAAAEELRAKGYEVHVYDRYDRMGGLLVYGIPGFKLEKSVVERRV
KLLADAGVIYHPNFEVGRDASLPELRRKHVAVLVATGVYKARDIKAPGSG
LGNIVAALDYLTTSNKVSLGDTVEAYENGSLNAAGKHVVVLGGGDTAMDC
VRTAIRQGATSVKCLYRRDRKNMPGSQREVAHAEEEGVEFIWQAAPEGFT
GDTVVTGVRAVRIHLGVADATGRQTPQVIEGSEFTVQADLVIKALGFEPE
DLPNAFDEPELKVTRWGTLLVDHRTKMTNMDGVFAAGDIVRGASLVVWAI
RDGRDAAEGIHAYAKAKAEA

3D structure

• PDB: 6s6s Cryo-EM Structures of Azospirillum brasilense Glutamate Synthase in Its Oligomeric Assemblies.
• Chain: E
• Resolution: 3.9 Å

Enzymatic activity

• Enzyme Commision number: 1.4.1.13: glutamate synthase (NADPH).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SF4	E	C48 S49 Q50 C51 C56 C109 V110 V119	C43 S44 Q45 C46 C51 C104 V105 V114
BS02	SF4	E	C60 C95 C99 C105 E125	C55 C90 C94 C100 E120
BS03	FAD	E	I98 G155 G157 P158 D178 R179 Y180 G185 L186 I191 V221 T241 Y244 D304 D443 S449	I93 G150 G152 P153 D173 R174 Y175 G180 L181 I186 V216 T236 Y239 D299 D438 S444

Gene Ontology

Molecular Function

• GO:0004355 glutamate synthase (NADPH) activity
• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding
• GO:0051536 iron-sulfur cluster binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0006537 glutamate biosynthetic process
• GO:0097054 L-glutamate biosynthetic process

External links

• PDB: RCSB:6s6s, PDBe:6s6s, PDBj:6s6s
• PDBsum: 6s6s
• PubMed: 31473159
• UniProt: Q05756|GLTD_AZOBR Glutamate synthase [NADPH] small chain (Gene Name=gltD)