Structure of PDB 6oay Chain E

Receptor sequence

>6oayE (length=570) Species: 83333 (Escherichia coli K-12)

LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGV
GKTAIVEGLAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLK
GVLNDLAKQEGNVILFIDELHTMAGNMLKPALARGELHCVGATTLDEYRQ
YIEKDAALERRFQKVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIV
AAATLSHRYIADRQLPDKAIDLIDEAASSIRMQIDSKRLLRNKVTDAEIA
EVLARWTGIPVSRMMESEREKLLRMEQELHHRVIGQNEAVDAVSNAIRRS
RAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFDSDEAMVRIDMSEF
MEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKAHPDVF
NILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHM
KELVLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLE
ERGYEIHISDEALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGE
LVPGKVIRLEVNEDRIVAVQ

3D structure

• PDB: 6oay Structural basis for substrate gripping and translocation by the ClpB AAA+ disaggregase.
• Chain: E
• Resolution: 3.3 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	E	K250 H641 G652 Y653	K90 H354 G365 Y366
BS02	AGS	E	V180 I181 G209 V210 G211 K212 T213 A214 I349 L353 I391	V20 I21 G49 V50 G51 K52 T53 A54 I178 L182 I220
BS03	AGS	E	R569 V570 I571 G608 V609 G610 K611 T612 E613 I774 Q778 R815 K818	R282 V283 I284 G321 V322 G323 K324 T325 E326 I487 Q491 R528 K531

Gene Ontology

Molecular Function

• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0016887 ATP hydrolysis activity
• GO:0042802 identical protein binding

Biological Process

• GO:0009408 response to heat
• GO:0034605 cellular response to heat
• GO:0042026 protein refolding

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0016020 membrane

External links

• PDB: RCSB:6oay, PDBe:6oay, PDBj:6oay
• PDBsum: 6oay
• PubMed: 31160557
• UniProt: P63284|CLPB_ECOLI Chaperone protein ClpB (Gene Name=clpB)