Structure of PDB 6oax Chain E

Receptor sequence

>6oaxE (length=579) Species: 83333 (Escherichia coli K-12)

LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGV
GKTAIVEGLAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLK
GVLNDLAKQEGNVILFIDELHTMVGAGKADGAMDAGNMLKPALARGELHC
VGATTLDEYRQYIEKDAALERRFQKVFVAEPSVEDTIAILRGLKERYELH
HHVQITDPAIVAAATLSHRYIADRQLPDKAIDLIDEAASSIRMQIDSKPE
NKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQELHHRVIGQNEAVD
AVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFDSDEA
MVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDE
VEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQER
FGELDYAHMKELVLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQ
LKRLYKRLEERGYEIHISDEALKLLSENGYDPVYGARPLKRAIQQQIENP
LAQQILSGELVPGKVIRLEVNEDRIVAVQ

3D structure

• PDB: 6oax Structural basis for substrate gripping and translocation by the ClpB AAA+ disaggregase.
• Chain: E
• Resolution: 2.9 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	E	K250 R252 K288 G652 Y653 V654	K90 R92 K128 G374 Y375 V376
BS02	AGS	E	D178 V180 I181 G209 G211 K212 T213 A214 I349 L353 I391	D18 V20 I21 G49 G51 K52 T53 A54 I189 L193 I231
BS03	AGS	E	R569 I571 G608 V609 G610 K611 T612 E613 N719 I774 Q778 R815 K818	R291 I293 G330 V331 G332 K333 T334 E335 N441 I496 Q500 R537 K540

Gene Ontology

Molecular Function

• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0016887 ATP hydrolysis activity
• GO:0042802 identical protein binding

Biological Process

• GO:0009408 response to heat
• GO:0034605 cellular response to heat
• GO:0042026 protein refolding

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0016020 membrane

External links

• PDB: RCSB:6oax, PDBe:6oax, PDBj:6oax
• PDBsum: 6oax
• PubMed: 31160557
• UniProt: P63284|CLPB_ECOLI Chaperone protein ClpB (Gene Name=clpB)