Structure of PDB 6nud Chain E

Receptor sequence

>6nudE (length=212) Species: 1308 (Streptococcus thermophilus) [Search protein sequence]

TFAKIKFSAQIRLETGLHIGGSDAFAAIGAIASPVIKDPITNIPIIPGSS
LKGKMRTLLAKVYNEKVAEKPSDDSDILSRLFGNSKDKRFKMGRLIFRDA
FLSNADELDSLGVRSYTEVKFENTIDRITAEANPRQIERAIRNSTFDFEL
IYEITDENENQVEEDFKVIRDGLKLLELDYLGGSGSRGYGKVAFEKLKAT
TVFGNYDVKTLN

3D structure

PDB

6nud Coupling of ssRNA cleavage with DNase activity in type III-A CRISPR-Csm revealed by cryo-EM and biochemistry.

Chain

Resolution

3.5 Å

3D
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Enzymatic activity

Enzyme Commision number

3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	E	G21 K53 G54 K55 R57 S73 F83 G84 N85 S86 K92 F122 E123 N124 T125 I126 E132 R136 Y181 G183 G184 S185 R188	G20 K52 G53 K54 R56 S72 F82 G83 N84 S85 K91 F121 E122 N123 T124 I125 E131 R135 Y180 G182 G183 S184 R187
BS02	rna	E	S86 N134 P135 R136 Q137	S85 N133 P134 R135 Q136

Gene Ontology

	Molecular Function
GO:0003723	RNA binding
GO:0004519	endonuclease activity

	Biological Process
GO:0051607	defense response to virus

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Molecular Function

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Biological Process

External links

PDB	RCSB:6nud, PDBe:6nud, PDBj:6nud
PDBsum	6nud
PubMed	30814678
UniProt	A0A0A7HIF0\|CSM3_STRTR CRISPR system Cms endoribonuclease Csm3 (Gene Name=csm3)

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