Structure of PDB 6je4 Chain E

Receptor sequence

>6je4E (length=1056) Species: 604162 (Neisseria meningitidis 8013)

AAFKPNSINYILGLAIGIASVGWAMVEIDEEENPIRLIDLGVRVFERAEV
PKTGDSLAMARRLARSVRRLTRRRAHRLLRTRRLLKREGVLQAANFDENG
LIKSLPNTPWQLRAAALDRKLTPLEWSAVLLHLIKHRGYLSQRKNELGAL
LKGVAGNAHALQTGDFRTPAELALNKFEKESGHIRNQRSDYSHTFSRKDL
QAELILLFEKQKEFGNPHVSGGLKEGIETLLMTQRPALSGDAVQKMLGHC
TFEPAEPKAAKNTYTAERFIWLTKLNNLRILEQGSERPLTDTERATLMDE
PYRKSKLTYAQARKLLGLEDTAFFKGLRYGKDNAEASTLMEMKAYHAISR
ALEKEGLKDKKSPLNLSPELQDEIGTAFSLFKTDEDITGRLKDRIQPEIL
EALLKHISFDKFVQISLKALRRIVPLMEQGKRYDEACAEIYGDNTEEKIY
LPPIPADEIRNPVVLRALSQARKVINGVVRRYGSPARIHIETAREVGKSF
KDRKEIEKRQEENRKDREKAAAKFREYFPNFVGEPKSKDILKLRLYEQQH
GKCLYSGKEINLGRLNEKGYVEIDAALPFSRTWDDSFNNKVLVLGSENQN
KGNQTPYEYFNGKDNSREWQEFKARVETSRFPRSKKQRILLQKFDEDGFK
ERNLNDTRYVNRFLCQFVADRMRLTGKGKKRVFASNGQITNLLRGFWGLR
KVRAENDRHHALDAVVVACSTVAMQQKITRFVRYKEMNALHQKTHFPQPW
EFFAQEVMIRVFGKPDGKPEFEEADTLEKLRTLLAEKLSSRPEAVHEYVT
PLFVSRAPNRKMSGQGHMETVKSAKRLDEGVSVLRVPLTQLKLKDLEKMV
NREREPKLYEALKARLEAHKDDPAKAFAEPFYKYDKAGNRTQQVKAVRVE
QVQKTGVWVRNHNGIADNATMVRVDVFEKGDKYYLVPIYSWQVAKGILPD
RAVVQGKDEEDWQLIDDSFNFKFSLHPNDLVEVITKKARMFGYFASCHRG
TGNINIRIHDLDHKIGKNGILEGIGVKTALSFQKYQIDELGKEIRPCRLK
KRPPVR

3D structure

• PDB: 6je4 Structures of Neisseria meningitidis Cas9 Complexes in Catalytically Poised and Anti-CRISPR-Inhibited States.
• Chain: E
• Resolution: 3.069 Å

Enzymatic activity

• Enzyme Commision number: 3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	E	Y140 R144 K145 G156 L158 L159 Y199 L246 M254 L255 K266 N285 R287 K333 K390 D418 K419 F420 R440 D442 R473 K656 F657 D658 Y672 R675 E845 T846 Q981 N1029 G1049 G1051 K1053 T1054	Y139 R143 K144 G148 L150 L151 Y191 L238 M246 L247 K258 N277 R279 K325 K382 D410 K411 F412 R432 D434 R460 K643 F644 D645 Y659 R662 E819 T820 Q955 N1003 G1023 G1025 K1027 T1028
BS02	dna	E	P52 K53 N944 T946 M947 Y965 H1024 R1025 R1033	P51 K52 N918 T920 M921 Y939 H998 R999 R1007
BS03	rna	E	S57 L58 A59 R62 R63 R66 R69 R70 L71 R73 R74 H77 R81 R83 R84 R88 N108 P110 W111 H133 K136 R138 G139 Q143 G190 H191 I192 R193 N194 Q195 R196 Q242 R243 A245 K269 F277 Q422 Y441 P466 R473 R479 R485 K486 R493 R665 T670 R675 P834 R836 K837 M838 G840 Q841 G842 H843 V847 S849 K851 R861 N877 R880 E881 K909 T917 Q918 Q919 K921 A922 V923 R924 R936 N939 W967 A970 K971 R1071 C1073 P1080 V1081 R1082	S56 L57 A58 R61 R62 R65 R68 R69 L70 R72 R73 H76 R80 R82 R83 R87 N107 P109 W110 H132 K135 R137 G138 Q142 G182 H183 I184 R185 N186 Q187 R188 Q234 R235 A237 K261 F269 Q414 Y433 P453 R460 R466 R472 K473 R480 R652 T657 R662 P808 R810 K811 M812 G814 Q815 G816 H817 V821 S823 K825 R835 N851 R854 E855 K883 T891 Q892 Q893 K895 A896 V897 R898 R910 N913 W941 A944 K945 R1045 C1047 P1054 V1055 R1056

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003723 RNA binding
• GO:0004519 endonuclease activity
• GO:0046872 metal ion binding

Biological Process

• GO:0043571 maintenance of CRISPR repeat elements
• GO:0051607 defense response to virus

External links

• PDB: RCSB:6je4, PDBe:6je4, PDBj:6je4
• PDBsum: 6je4
• PubMed: 31668930
• UniProt: C9X1G5|CAS9_NEIM8 CRISPR-associated endonuclease Cas9 (Gene Name=cas9)