Structure of PDB 5y58 Chain E

Receptor sequence
>5y58E (length=548) Species: 559292 (Saccharomyces cerevisiae S288C) [Search protein sequence]
IHEGILFCIELSETMFKESSDLEYKSPLLEILESLDELMSQLVITRPGTA
IGCYFYYCNREDAKEGIYELFPLRDINATFMKKLNDLLEDLSSGRISLYD
YFMFQQTGSEKQVRLSVLFTFMLDTFLEEIPGQKQLSNKRVFLFTDIDKP
QEAQDIDERARLRRLTIDLFDNKVNFATFFIGYADKPFDNEFYSDILQLG
DSEFDGPSTKPIDAKYIKSRILRKKEVKRIMFQCPLILDEKTNFIVGVKG
YTMYTHEKAGVRYKLVYEHEDIRQEAYSKRKFLNPITGEDVTGKTVKVYP
YGDLDINLSDSQDQIVMEAYTQKDAFLKIIGFRSSSKSIHYFNNIDKSSF
IVPDEAKYEGSIRTLASLLKILRKKDKIAILWGKLKSNSHPSLYTLSPSS
VKDYNEGFYLYRVPFLDEIRKFPSLLSYDDGSEHKLDYDNMKKVTQSIMG
YFNLRDGYNPSDFKNPLLQKHYKVLHDYLLQIETTFDENETPNTKKDRMM
REDDSLRKLYYIRNKILESEKSEDPIIQRLNKYVKIWNMFYKKFNDDN
3D structure
PDB5y58 Structural Insights into Yeast Telomerase Recruitment to Telomeres
ChainE
Resolution2.8 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.6.4.12: DNA helicase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 rna E R122 R537 R543 Y547 R95 R501 R507 Y511 PDBbind-CN: Kd=75nM
BS02 rna E H292 E293 K294 V297 R298 Y299 R316 K333 N424 S425 H426 D453 R456 H256 E257 K258 V261 R262 Y263 R280 K297 N388 S389 H390 D417 R420 PDBbind-CN: Kd=75nM
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003678 DNA helicase activity
GO:0003684 damaged DNA binding
GO:0003690 double-stranded DNA binding
GO:0004386 helicase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016787 hydrolase activity
GO:0016887 ATP hydrolysis activity
GO:0042162 telomeric DNA binding
GO:0070034 telomerase RNA binding
Biological Process
GO:0000723 telomere maintenance
GO:0000724 double-strand break repair via homologous recombination
GO:0000725 recombinational repair
GO:0000727 double-strand break repair via break-induced replication
GO:0006281 DNA repair
GO:0006303 double-strand break repair via nonhomologous end joining
GO:0006310 DNA recombination
GO:0006325 chromatin organization
GO:0030466 silent mating-type cassette heterochromatin formation
GO:0031509 subtelomeric heterochromatin formation
GO:0032508 DNA duplex unwinding
GO:0097552 mitochondrial double-strand break repair via homologous recombination
GO:0097695 establishment of protein-containing complex localization to telomere
Cellular Component
GO:0000781 chromosome, telomeric region
GO:0005634 nucleus
GO:0005635 nuclear envelope
GO:0005694 chromosome
GO:0043564 Ku70:Ku80 complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:5y58, PDBe:5y58, PDBj:5y58
PDBsum5y58
PubMed29290466
UniProtP32807|KU70_YEAST ATP-dependent DNA helicase II subunit 1 (Gene Name=YKU70)

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