Structure of PDB 5xuz Chain E

Receptor sequence

>5xuzE (length=1208) Species: 1410628 (Lachnospiraceae bacterium ND2006)

GSHMSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAEDY
KGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENL
EINLRKEIAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGF
TTAFTGFFDNRENMFSEEAKSTSIAFRCINENLTRYISNMDIFEKVDAIF
DKHEVQEIKEKILNSDYDVEDFFEGEFFNFVLTQEGIDVYNAIIGGFVTE
SGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVLSDRESLSFYGEGYTSD
EEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKNGPAISTI
SKDIFGEWNVIRDKWNAEYDDIHLVTEKYEDDRRKSFKKIGSFSLEQLQE
YADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAV
VAIMKDLLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDH
IYDAIRNYVTQKPYSKDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSK
YYLAIMDKKYAKCLQKIDKDDVNGNYEKINYKLLPGPNKMLPKVFFSKKW
MAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKLIDFFKDSISRYPKWSNA
YDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLVEEGKLYMF
QIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLK
KEELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAI
NKCPKNIFKINTEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVE
QYSLNEIINNFNGIRIKTDYHSLLDKKEKERFEARQNWTSIENIKELKAG
YISQVVHKICELVEKYDAVIALEDLNVKVEKQVYQKFEKMLIDKLNYMVD
KKSNPCATGGALKGYQITNKFESFKSMSTQNGFIFYIPAWLTSKIDPSTG
FVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALDYKNFSRTDADY
IKKWKLYSYGNRIRIFDWEEVCLTSAYKELFNKYGINYQQGDIRALLCEQ
SDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRNYE
AQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEW
LEYAQTSV

3D structure

• PDB: 5xuz Structural Basis for the Canonical and Non-canonical PAM Recognition by CRISPR-Cpf1.
• Chain: E
• Resolution: 2.4 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	E	S14 K15 T16 R18 F19 K20 K51 N157 R158 R174 K253 L261 Q264 Y277 K278 L281 W355 R386 K390 K464 D505 R508 K520 N706 K707 S710 K712 H714 G715 T716 N718 H720 E743 R747 H759 I765 N767 K768 N769 N772 K774 T777 L779 Y781 V783 Y784 K785 R788 Q793 H797 F863 Y872 R887 Q888 E898 G902 S905 H909 K953 K960	S17 K18 T19 R21 F22 K23 K54 N160 R161 R177 K256 L264 Q267 Y280 K281 L284 W358 R384 K388 K462 D503 R506 K518 N704 K705 S708 K710 H712 G713 T714 N716 H718 E741 R745 H757 I763 N765 K766 N767 N770 K772 T775 L777 Y779 V781 Y782 K783 R786 Q791 H795 F861 Y870 R885 Q886 E896 G900 S903 H907 K944 K951
BS02	dna	E	N160 K167 S168 G242 V245 K251 N256 E257 N260 K272 S286 S288 Y290 K349 W355 N509 T512 Q513 K514 D535 K538 Y542 L585 P587 M592 S599 K600 K601 Y646 K648 W649 S739 G740 N895 I896 K897 K945 S982 F983 K984	N163 K170 S171 G245 V248 K254 N259 E260 N263 K275 S289 S291 Y293 K352 W358 N507 T510 Q511 K512 D533 K536 Y540 L583 P585 M590 S597 K598 K599 Y644 K646 W647 S737 G738 N893 I894 K895 K936 S973 F974 K975
BS03	dna	E	K121 G146 F147 T148 T149 K560 K591 K595	K124 G149 F150 T151 T152 K558 K589 K593

Gene Ontology

Molecular Function

• GO:0046872 metal ion binding

External links

• PDB: RCSB:5xuz, PDBe:5xuz, PDBj:5xuz
• PDBsum: 5xuz
• PubMed: 28781234
• UniProt: A0A182DWE3