Structure of PDB 5xf9 Chain E

Receptor sequence

>5xf9E (length=567) Species: 297 (Hydrogenophilus thermoluteolus)

TTERQRTAPGLLAALHQARSPLDAQALAELSTAFSLPPGEIAATASFYHF
FQTPPARYQIHFVDHVVDHHAGVAALCNHLCAAFAIQPGQRTADARLFVG
WTACAGLSDQAPAALINGRPMPRLDAARIDALIEKIQAQIPMDQWPTEWF
AVTNAIHRHGPLLTWLDTTPAEAVFEHPTAHDPDAILQAVTDAGLRGRGG
AGFPTATKWRFCRENADPERFLICNADEGEPGTFKDRVLLTRYPEHLFAG
MILAARAIGADKAILYLRYEYQYLLPQLEAARERIASAERVTLEIALGAG
AYVCGEESALIESLEGKPGRPRVRPPYPVTQGYLGHPTVVNNVETLVAVA
AIVGNGAAWWRALGTPDSSGPKLFCVSGDVAQPGLYEFPYGVALGDVVTA
ARPLGTRYAVQVSGPSGTLLPATPEQLARPLAFEALPCNGTVMVFDVRRD
PVAIVHHFARFFAHESCGFCTPCRVGTQLIAKTFEKIAAGYATRFDLERL
APALEAMRLASNCGFGLSAGNPVRDLIAHFRQQLEAQLQPHDFIPAFSLD
AELAATRHLAQFEQPEV

3D structure

• PDB: 5xf9 Structural basis of the redox switches in the NAD(+)-reducing soluble [NiFe]-hydrogenase
• Chain: E
• Resolution: 2.58 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FMN	E	G202 G204 K213 N230 D232 Y314 G317 E318 E319 V352 N353 N354 T357 G526 F527 E585	G197 G199 K208 N225 D227 Y302 G305 E306 E307 V340 N341 N342 T345 G514 F515 E563
BS02	SF4	E	P333 S478 C479 G480 F481 C482 C485 S523 N524 C525 F527	P321 S466 C467 G468 F469 C470 C473 S511 N512 C513 F515

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0008137 NADH dehydrogenase (ubiquinone) activity
• GO:0010181 FMN binding
• GO:0046872 metal ion binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:1902600 proton transmembrane transport

External links

• PDB: RCSB:5xf9, PDBe:5xf9, PDBj:5xf9
• PDBsum: 5xf9
• PubMed: 28860386
• UniProt: A0A077L6X8