Structure of PDB 5utu Chain E

Receptor sequence

>5utuE (length=494) Species: 353152 (Cryptosporidium parvum Iowa II)

AYKMESRIKDISLAEFGLQDMEIAKTDMMGLVELQRKYRDSKPLKGARIT
GSLHLTIETSVLVETLYELGAEIRWCSCNIYSTQDHAAAALVKKNIATVF
AWKNETIEDYWVCLNDAMTWRNPNDKICGPNLIVDDGGDATLILHEGVKA
EIEYEKYNKIPEYLETELDENGKQLSMDLKCMYKVLKMELLKNPFRWRGM
LKDLYGVSEETTTGVLRLKIMESEGKLLLPAINVNDSVTKSKFDNTYGCR
QSLLHGLFNGCIQMLAGKKIVVLGYGEVGKGCAQGLSGVGARVIVTEIDP
ICALQASMEGYQVSVLEDVVSEADIFITATGNKDVITVEHMRKMKENAYI
ANIGHFDDEIDVYGLENYPGIKVIEVKQNVHKFTFPDTQKSVILLCKGRL
VNLGCATGHPPLVMSMSFTNQVLAQMDLWKSRELVDRSKNTRFFVKKLSK
ELDEYVARLHLDVLGIKLTKLTETQAKYINVSINGPYKSEDYRY

3D structure

• PDB: 5utu 2.65 Angstrom Resolution Crystal Structure of S-adenosylhomocysteinase from Cryptosporidium parvum in Complex with SAH and NAD
• Chain: E
• Resolution: 2.65 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H53 S76 S81 D137 E211 N236 K241 D245 N246 C250 H356 H410 S418 Q422
• Catalytic site (residue number reindexed from 1): H54 S77 S82 D136 E210 N235 K240 D244 N245 C249 H355 H409 S417 Q421
• Enzyme Commision number: 3.13.2.1: adenosylhomocysteinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAD	E	N246 C250 G277 E278 V279 E298 I299 T331 N333 I354 G355 N403 H410	N245 C249 G276 E277 V278 E297 I298 T330 N332 I353 G354 N402 H409
BS02	SAH	E	L52 H53 T55 E57 C77 N78 D137 T212 D245 H356 F357 L401 H410 M415	L53 H54 T56 E58 C78 N79 D136 T211 D244 H355 F356 L400 H409 M414
BS03	NAD	E	K489 Y493	K488 Y492

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0004013 adenosylhomocysteinase activity
• GO:0016787 hydrolase activity

Biological Process

• GO:0006730 one-carbon metabolic process
• GO:0033353 S-adenosylmethionine cycle

Cellular Component

• GO:0005829 cytosol

External links

• PDB: RCSB:5utu, PDBe:5utu, PDBj:5utu
• PDBsum: 5utu
• UniProt: Q5CPH1