Structure of PDB 5ola Chain E

Receptor sequence

>5olaE (length=989) Species: 9606 (Homo sapiens)

QLSGQQQRLLAFFKCCLLTDQLPLAHHLLVVHHGQRQKRKLLTLDMYNAV
MLGWARQGAFKELVYVLFMVKDAGLTPDLLSYAAALQCMGRQDQDAGTIE
RCLEQMSQEGLKLQALFTAVLLSEEDRATVLKAVHKVKPTFSLPPQLPPP
VNTSKLLRDVYAKDGRVSYPKLHLPLKTLQCLFEKQLHMELASRVCVVSV
EKPTLPSKEVKHARKTLKTLRDQWEKALCRALRETKNRLEREVYEGRFSL
YPFLCLLDEREVVRMLLQVLQALPAQGESFTTLARELSARTFSRHVVQRQ
RVSGQVQALQNHYRKYLCLLASDAEVPEPCLPRQYWEALGAPEALREQPW
PLPVQMELGKLLAEMLVQATQMPCSLDRSSRLVPVLYHVYSFRNVQQIGI
LKPHPAYVQLLEKAAEPTLTFEAVDVPMLCPPLPWTSPHSGAFLLSPTKL
MRTVEGATQHQELLETCPPTALHGALDALTQLGNCAWRVNGRVLDLVLQL
FQAKGCPQLGVPAPPSEAPQPPEAHLPHSAAPARKAELRRELAHCQKVAR
EMHSLRAEALYRLSLAQHLRDRVFWLPHNMDFRGRTYPCPPHFNHLGSDV
ARALLEFAQGRPLGPHGLDWLKIHLVNLTGLKKREPLRKRLAFAEEVMDD
ILDSADQPLTGRKWWMGAEEPWQTLACCMEVANAVRASDPAAYVSHLPVH
QDGSCNGLQHYAALGRDSVGAASVNLEPSDVPQDVYSGVAAQVEVFRRQD
AQRGMRVAQVLEGFITRKVVKQTVMTVVYGVTRYGGRLQIEKRLRELSDF
PQEFVWEASHYLVRQVFKSLQEMFSGTRAIQHWLTESARLISHMGSVVEW
VTPLGVPVIQPYRLDSRKPNTRKQKNGFPPNFIHSLDSSHMMLTALHCYR
KGLTFVSVHDCYWTHAADVSVMNQVCREQFVRLHSEPILQDLSRFLVKRF
CSEPQKILEASQLKETLQAVPKPGAFDLEQVKRSTYFFS

3D structure

• PDB: 5ola Mechanism of Transcription Anti-termination in Human Mitochondria.
• Chain: E
• Resolution: 3.904 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.6: DNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	E	W1026 T1112 R1113	W806 T871 R872
BS02	rna	E	R613 N614 E771 S774 L775 R805 L816 G817 R822 V1149 H1150 D1151	R393 N394 E551 S554 L555 R585 L596 G597 R602 V908 H909 D910
BS03	dna	E	R672 H745 P747 H748 H773 S774 Y781 F802 R803 Y807 P811 H812 Y999 G1000 V1001 T1002 Y1004 G1005 Q1009 Y1082 R1113	R452 H525 P527 H528 H553 S554 Y561 F582 R583 Y587 P591 H592 Y779 G780 V781 T782 Y784 G785 Q789 Y862 R872

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003899 DNA-directed 5'-3' RNA polymerase activity

Biological Process

• GO:0006351 DNA-templated transcription

External links

• PDB: RCSB:5ola, PDBe:5ola, PDBj:5ola
• PDBsum: 5ola
• PubMed: 29033127
• UniProt: O00411|RPOM_HUMAN DNA-directed RNA polymerase, mitochondrial (Gene Name=POLRMT)