Structure of PDB 5nsq Chain E

Receptor sequence

>5nsqE (length=519) Species: 185431 (Trypanosoma brucei brucei TREU927)

TLPKAEAKELSAFVQSCVEYKTNVCFTDVAAYESNQKGVLSSGLAVLVGT
HKQLRDPAVQRLPFYNPAVAEAIERVKEGGTYGVLVEGLANAAGSKFVRV
VVGEVPTKASRNNCPARPDVVTALVTAALDEVKEPNTTVDVFVLSNAVLP
IAAAVARCGKHNFSAKDGAAAAAYNSGKVSRLQVVFPEPPAIPPKDLEAV
ATSTQLCQRLVDAPPNLLTTATFTEIAQGYAKALGFDVDVICGDDLCERG
YGGIYSVGKAAFEAPRLVTLLYTPKGTPVKKVSLVGKGIVYDCGGLALKP
ADYMKLMKHDMGGAAAVFCGFLTAVRLQQPVQLSCTLCLAENAIGPKSYR
NDDIIVMKSGKTVEVINTDAEGRIVLGDGVFHATNELSFTPDVVIDMATL
TGAQGIATGRHHAGLYVNEEGAEAAMLRAGRESGETCFPVLYCPEYHEPE
FKSNHADMTNLMERRDNAGVSCAGYFITTHLSPKFTGAHIHVDLAYPVFN
SNGATGFGPALLTEYFRKL

3D structure

• PDB: 5nsq Structural Characterization of Acidic M17 Leucine Aminopeptidases from the TriTryps and Evaluation of Their Role in Nutrient Starvation inTrypanosoma brucei.
• Chain: E
• Resolution: 3.0 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): K301 R375
• Catalytic site (residue number reindexed from 1): K299 R373

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MN	E	K289 D294 D312 E373	K287 D292 D310 E371
BS02	MN	E	D294 D371 E373	D292 D369 E371
BS03	BB2	E	K289 D294 K301 N369 D371 E373 A405	K287 D292 K299 N367 D369 E371 A403

Gene Ontology

Molecular Function

• GO:0004177 aminopeptidase activity
• GO:0005515 protein binding
• GO:0008233 peptidase activity
• GO:0030145 manganese ion binding
• GO:0046872 metal ion binding
• GO:0070006 metalloaminopeptidase activity

Biological Process

• GO:0006508 proteolysis
• GO:0019538 protein metabolic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:5nsq, PDBe:5nsq, PDBj:5nsq
• PDBsum: 5nsq
• PubMed: 28815215
• UniProt: Q385B0