Structure of PDB 5ng6 Chain E

Receptor sequence

>5ng6E (length=1262) Species: 401614 (Francisella tularensis subsp. novicida U112)

NASIYQEFVNKYSLSKTLRFELIPQGKTLENIKARGLILDDEKRAKDYKK
AKQIIDKYHQFFIEEILSSVCISEDLLQNYSDVYFKLKKSDDDNLQKDFK
SAKDTIKKQISEYIKDSEKFKNLFNQNLIDAKKGQESDLILWLKQSKDNG
IELFKANSDITDIDEALEIIKSFKGWTTYFKGFHENRKNVYSSNDIPTSI
IYRIVDDNLPKFLENKAKYESLKDKAPEAINYEQIKKDLAEELTFDIDYK
TSEVNQRVFSLDEVFEIANFNNYLNQSGITKFNTIIGGKFVNGENTKRKG
INEYINLYSQQINDKTLKKYKMSVLFKQILSDDKLEDDSDVVTTMQSFYE
QIAAFKTVEEKSIKETLSLLFDDLKAQKLDLSKIYFKNDKSLTDLSQQVF
DDYSVIGTAVLEYITQQIAQELIAKKTEKAKYLSLETIKLALEEFNKHRD
IDKQCRFEEILANFAAIPMIFDEIAQNKDNLAQISIKYQNQGKKDLLQAS
AEDDVKAIKDLLDQTNNLLHKLKIFHISQSEDKANILDKDEHFYLVFEEC
YFELANIVPLYNKIRNYITQKPYSDEKFKLNFENSTLANGWDKNKEPDNT
AILFIKDDKYYLGVMNKKNNKIFDDKAIKENKGEGYKKIVYKLLPGANKM
LPKVFFSAKSIKFYNPSEDILRIRNHSTHTKNGSPQKGYEKFEFNIEDCR
KFIDFYKQSISKHPEWKDFGFRFSDTQRYNSIDEFYREVENQGYKLTFEN
ISESYIDSVVNQGKLYLFQIYNKDFSAYSKGRPNLHTLYWKALFDERNLQ
DVVYKLNGEAELFYRKQSIPKKITHPAKEAIANKNKDNPKKESVFEYDLI
KDKRFTEDKFFFHCPITINFKSSGANKFNDEINLLLKEKANDVHILSIDR
GERHLAYYTLVDGKGNIIKQDTFNIIGNDRMKTNYHDKLAAIERKDWKKI
NNIKEMKEGYLSQVVHEIAKLVIEYNAIVVFEDLNFGFKRGRFKVEKQVY
QKLEKMLIEKLNYLVFKDNEFDKTGGVLRAYQLTAPFETFKKMGKQTGII
YYVPAGFTSKICPVTGFVNQLYPKYESVSKSQEFFSKFDKICYNLDKGYF
EFSFDYKNFAAKGKWTIASFGSRLINNWDTREVYPTKELEKLLKDYSIEY
GHGECIKAAICGEKKFFAKLTSVLNTILQMRNSKTGTELDYLISPVADVN
GNFFDSRQAPKNMPQDADANGAYHIGLKGLMLLGRIKNNQEGKKLNLVIK
NEEYFEFVQNRN

3D structure

• PDB: 5ng6 Structural Basis for Guide RNA Processing and Seed-Dependent DNA Targeting by CRISPR-Cas12a.
• Chain: E
• Resolution: 3.342 Å

Enzymatic activity

• Enzyme Commision number: 3.1.21.1: deoxyribonuclease I.
  4.6.1.22: Bacillus subtilis ribonuclease.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	E	S14 K15 T16 R18 F19 Y47 K51 G181 N185 K589 Y591 K595 N790 K791 S794 Y796 G799 R800 N802 L803 H804 R833 H843 I849 A850 N851 K852 N853 N856 S861 V862 F863 Y865 I868 K869 D870 K871 R872 H881 R948 K956 G983 S986 Q987 H990 K1034 K1041	S15 K16 T17 R19 F20 Y48 K52 G182 N186 K571 Y573 K577 N772 K773 S776 Y778 G781 R782 N784 L785 H786 R815 H825 I831 A832 N833 K834 N835 N838 S843 V844 F845 Y847 I850 K851 D852 K853 R854 H863 R930 K938 G959 S962 Q963 H966 K1010 K1017

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003723 RNA binding
• GO:0004519 endonuclease activity
• GO:0004530 deoxyribonuclease I activity
• GO:0016829 lyase activity

Biological Process

• GO:0006259 DNA metabolic process
• GO:0051607 defense response to virus

External links

• PDB: RCSB:5ng6, PDBe:5ng6, PDBj:5ng6
• PDBsum: 5ng6
• PubMed: 28431230
• UniProt: A0Q7Q2|CS12A_FRATN CRISPR-associated endonuclease Cas12a (Gene Name=cas12a)