Structure of PDB 5mlc Chain E

Receptor sequence

>5mlcE (length=219) Species: 3562 (Spinacia oleracea)

SVDAGIGVMGTKLGMMSFFEEDGTVVPVTVIGFKEGNIVTQVKTESTDGY
NAVQVGYERLRDRKLTMPERGHLNKAGVIPMRHLQEFRLVSVDDFTPSQK
LLFEELFKEGDMVDISGTTIGKGFQGGIKRHNFKRGLMTHGSKSHRALGS
IGAGTTPGHVYKGKKMPGRMGGTKTKIRKLKIMKIDTDLRVVMIKGAVPG
KPGNLLRLAPAKIVGKNIP

3D structure

• PDB: 5mlc Cryo-EM structure of the spinach chloroplast ribosome reveals the location of plastid-specific ribosomal proteins and extensions.
• Chain: E
• Resolution: 3.6 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	E	K96 M99 P111 Q125 D132 Y134 Q138 P152 E153 H156 K159 L168 Q169 E170 R172 I204 K206 F208 G210 K213 R214 F217 K218 R219 M222 T223 H224 G225 S226 K227 S228 H229 R230 L232 G233 S234 I235 G236 A237 G238 T239 T240 P241 H243 V244 Y245 K246 G247 K248 M250 P251 K258 K260 I261 R262 K263 L264 A281 V282 G284 K285 K296 V298 P303	K12 M15 P27 Q41 D48 Y50 Q54 P68 E69 H72 K75 L84 Q85 E86 R88 I120 K122 F124 G126 K129 R130 F133 K134 R135 M138 T139 H140 G141 S142 K143 S144 H145 R146 L148 G149 S150 I151 G152 A153 G154 T155 T156 P157 H159 V160 Y161 K162 G163 K164 M166 P167 K174 K176 I177 R178 K179 L180 A197 V198 G200 K201 K212 V214 P219
BS02	rna	E	T150 M151 R166 G205 G207 F208 R253 K285 P286	T66 M67 R82 G121 G123 F124 R169 K201 P202

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0009507 chloroplast
• GO:0009941 chloroplast envelope
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:5mlc, PDBe:5mlc, PDBj:5mlc
• PDBsum: 5mlc
• PubMed: 27986857
• UniProt: P82191|RK3_SPIOL Large ribosomal subunit protein uL3c (Gene Name=RPL3)