Structure of PDB 5k77 Chain E

Receptor sequence
>5k77E (length=349) Species: 5759 (Entamoeba histolytica) [Search protein sequence]
QIQHIAIVGCVHGKYREMYRQLSEYEKSTGKEISFVICTGDMQTLRYEAD
LVYLKVPPKYKQMGDFHLYYEGKEKAPYLTLFIGGNAESSNVLLHLYNGG
FVCFNMYYLGVCSCININGLRIVGVSGIYKSFDEKKPYTYPPSPNDVVSL
FHTRNYVIQMLSNLSQSSQIDISLSHDWPQGIVMKGNYKQLYRFQPGFKK
DGASLGSPINKVILNTLKPKYWISGHMHCEYHAEEGPTHFIALGKIGYKN
AISYLDLPLKQKTDLEYDKDWVCNLIMTWPAFSNKAQFPDLSYSISELLS
KRTKELDKKIIELWEKYIGLKIIYDSDTFDIQFTSRRFYIEKIYNELNI
3D structure
PDB5k77 Metal dependence and branched RNA cocrystal structures of the RNA lariat debranching enzyme Dbr1.
ChainE
Resolution2.17 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.4.-
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 rna E H16 Q47 P61 Y64 I132 K134 F155 H156 K249 H12 Q43 P57 Y60 I128 K130 F151 H152 K245
BS02 rna E H16 N90 F202 D205 L209 H230 M231 H232 H12 N86 F198 D201 L205 H226 M227 H228
BS03 FE2 E D45 N90 H180 H230 D41 N86 H176 H226
BS04 ZN E C14 H16 H232 C10 H12 H228
Gene Ontology
Molecular Function
GO:0003723 RNA binding
GO:0005506 iron ion binding
GO:0008270 zinc ion binding
GO:0008419 RNA lariat debranching enzyme activity
GO:0016787 hydrolase activity
GO:0030145 manganese ion binding
GO:0046872 metal ion binding
Biological Process
GO:0000375 RNA splicing, via transesterification reactions
GO:0000398 mRNA splicing, via spliceosome
GO:0006397 mRNA processing
Cellular Component
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0048471 perinuclear region of cytoplasm

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Cellular Component
External links
PDB RCSB:5k77, PDBe:5k77, PDBj:5k77
PDBsum5k77
PubMed27930312
UniProtC4M1P9|DBR1_ENTH1 Lariat debranching enzyme (Gene Name=DBR1)

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