Structure of PDB 5hm8 Chain E

Receptor sequence

>5hm8E (length=489) Species: 353152 (Cryptosporidium parvum Iowa II)

KMESRIKDISLAEFGLQDMEIAKTDMMGLVELQRKYRDSKPLKGARITGS
LHLTIETSVLVETLYELGAEIRWCSCNIYSTQDHAAAALVKKNIATVFAW
KNETIEDYWVCLNDAMTWRNPICGPNLIVDDGGDATLILHEGVKAEIEYE
KYNKIPEYLETELDENGKQLSMDLKCMYKVLKMELLKNPFRWRGMLKDLY
GVSEETTTGVLRLKIMESEGKLLLPAINVNDSVTKSKFDNTYGCRQSLLH
GLFNGCIQMLAGKKIVVLGYGEVGKGCAQGLSGVGARVIVTEIDPICALQ
ASMEGYQVSVLEDVVSEADIFITATGNKDVITVEHMRKMKENAYIANIGH
FDDEIDVYGLENYPGIKVIEVKQNVHKFTFPDTQKSVILLCKGRLVNLGC
ATGHPPLVMSMSFTNQVLAQMDLWKSRELVDRSKNTRFFVKKLSKELDEY
VARLHLDVLGIKLTKLTETQAKYINVSINGPYKSEDYRY

3D structure

• PDB: 5hm8 2.85 Angstrom Crystal Structure of S-adenosylhomocysteinase from Cryptosporidium parvum in Complex with Adenosine and NAD.
• Chain: E
• Resolution: 2.85 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H53 S76 S81 D137 E211 N236 K241 D245 N246 C250 H356 H410 S418 Q422
• Catalytic site (residue number reindexed from 1): H52 S75 S80 D131 E205 N230 K235 D239 N240 C244 H350 H404 S412 Q416
• Enzyme Commision number: 3.13.2.1: adenosylhomocysteinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ADN	E	H53 T55 E57 D137 E211 T212 D245 H356 G409 H410 M415	H52 T54 E56 D131 E205 T206 D239 H350 G403 H404 M409
BS02	NAD	E	T212 T213 T214 N246 G275 E278 V279 E298 I299 T331 N333 I354 G355 H356 N403 H410	T206 T207 T208 N240 G269 E272 V273 E292 I293 T325 N327 I348 G349 H350 N397 H404
BS03	NAD	E	K489 Y493	K483 Y487

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0004013 adenosylhomocysteinase activity
• GO:0016787 hydrolase activity

Biological Process

• GO:0006730 one-carbon metabolic process
• GO:0033353 S-adenosylmethionine cycle

Cellular Component

• GO:0005829 cytosol

External links

• PDB: RCSB:5hm8, PDBe:5hm8, PDBj:5hm8
• PDBsum: 5hm8
• UniProt: Q5CPH1