Structure of PDB 5f9h Chain E

Receptor sequence

>5f9hE (length=638) Species: 9606 (Homo sapiens)

KPRNYQLELALPAMKGKNTIICAPTGCGKTFVSLLICEHHLKKFPQGQKG
KVVFFANQIPVYEQQKSVFSKYFERHGYRVTGISGATAENVPVEQIVENN
DIIILTPQILVNNLKKGTIPSLSIFTLMIFDECHNTSKQHPYNMIMFNYL
DQKLGGSSGPLPQVIGLTASVGVGDAKNTDEALDYICKLCASLDASVIAT
VKHNLEELEQVVYKPQKFFRKVESRIDKFKYIIAQLMRDTESLAKRICRE
FGTQKYEQWIVTVQKACMVFQMPDKDEESRICKALFLYTSHLRKYNDALI
ISEHARMKDALDYLKDFFSNVFDEIEQDLTQRFEEKLQELESVSRDPSNE
NPKLEDLCFILQEEYHLNPETITILFVKTRALVDALKNWIEGNPKLSFLK
PGILTGGDHNILIATSVADEGIDIAQCNLVILYEYVGNVIKMIQTRGRGR
ARGSKCFLLTSNAGVIEKEQINMYKEKMMNDSILRLQTWDEAVFREKILH
IQTHEKFIRDSQEPKPVPDKENKKLLCRKCKALACYTADVRVIEECHYTV
LGDAFKECFVSRPHPKPKQFSSFEKRAKIFCARQNCSHDWGIHVKYKTFE
IPVIKIESFVVEDIATVQTLYSKWKDFHFEKIPFDPAE

3D structure

• PDB: 5f9h Structural basis for m7G recognition and 2'-O-methyl discrimination in capped RNAs by the innate immune receptor RIG-I.
• Chain: E
• Resolution: 3.1 Å

Enzymatic activity

• Enzyme Commision number: 3.6.4.13: RNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	E	I300 S325 G326 T347 Q349 K379 Q380 H381 Q507 E510 Q511 R546 K635 T636 R637 G663 T697 S698 V718 N720 F853 K888	I59 S84 G85 T106 Q108 K138 Q139 H140 Q254 E257 Q258 R293 K378 T379 R380 G406 T415 S416 V436 N438 F570 K605	PDBbind-CN: Kd=1.8nM
BS02	ZN	E	C810 C813 C864 C869	C527 C530 C581 C586
BS03	GTP	E	H830 H847 F853 K858 K861 I875 K888	H547 H564 F570 K575 K578 I592 K605

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0005524 ATP binding

External links

• PDB: RCSB:5f9h, PDBe:5f9h, PDBj:5f9h
• PDBsum: 5f9h
• PubMed: 26733676
• UniProt: O95786|RIGI_HUMAN Antiviral innate immune response receptor RIG-I (Gene Name=RIGI)