Structure of PDB 5f9f Chain E

Receptor sequence

>5f9fE (length=672) Species: 9606 (Homo sapiens)

KPRNYQLELALPAMKGKNTIICAPTGCGKTFVSLLICEHHLKKFPQGQKG
KVVFFANQIPVYEQQKSVFSKYFERHGYRVTGISGATAENVPVEQIVENN
DIIILTPQILVNNLKKGTIPSLSIFTLMIFDECHNTSKQHPYNMIMFNYL
DQKLGGSSGPLPQVIGLTASVGVGDAKNTDEALDYICKLCASLDASVIAT
VKHNLEELEQVVYKPQKFFRKVESRISDKFKYIIAQLMRDTESLAKRICK
DREFGTQKYEQWIVTVQKACMVFQMPDKDEESRICKALFLYTSHLRKYND
ALIISEHARMKDALDYLKDFFSNVRAAGFDEIEQDLTQRFEEKLQELESV
SRDPSNENPKLEDLCFILQEEYHLNPETITILFVKTRALVDALKNWIEGN
PKLSFLKPGILTGRGKTNQNTGMTLPAQKCILDAFKASGDHNILIATSVA
DEGIDIAQCNLVILYEYVGNVIKMIQTRGRGRARGSKCFLLTSNAGVIEK
EQINMYKEKMMNDSILRLQTWDEAVFREKILHIQTHEKFIRDSQEKPKPV
PDKENKKLLCRKCKALACYTADVRVIEECHYTVLGDAFKECFVSRPHPKP
KQFSSFEKRAKIFCARQNCSHDWGIHVKYKTFEIPVIKIESFVVEDIATG
VQTLYSKWKDFHFEKIPFDPAE

3D structure

• PDB: 5f9f Structural basis for m7G recognition and 2'-O-methyl discrimination in capped RNAs by the innate immune receptor RIG-I.
• Chain: E
• Resolution: 2.601 Å

Enzymatic activity

• Enzyme Commision number: 3.6.4.13: RNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	E	N298 I300 S325 G326 T347 Q349 K379 Q380 H381 Q507 E510 Q511 V514 R546 K635 T636 R637 T662 G663 T667 N668 T697 S698 V718 N720 K750 H830 F853 K858 I875 V886 K888	N57 I59 S84 G85 T106 Q108 K138 Q139 H140 Q257 E260 Q261 V264 R296 K385 T386 R387 T412 G413 T417 N418 T447 S448 V468 N470 K500 H580 F603 K608 I625 V636 K638	PDBbind-CN: Kd=38.5nM
BS02	ZN	E	C810 C864 C869	C560 C614 C619
BS03	BU3	E	I262 A264 K270 V273 T409	I21 A23 K29 V32 T168
BS04	BU3	E	Y717 G719 N720 K723 M724 T727 E751	Y467 G469 N470 K473 M474 T477 E501

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0005524 ATP binding

External links

• PDB: RCSB:5f9f, PDBe:5f9f, PDBj:5f9f
• PDBsum: 5f9f
• PubMed: 26733676
• UniProt: O95786|RIGI_HUMAN Antiviral innate immune response receptor RIG-I (Gene Name=RIGI)