Structure of PDB 5dqt Chain E

Receptor sequence
>5dqtE (length=93) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
MSMLVVVTENVPPRLRGRLAIWLLEVRAGVYVGDVSAKIREMIWEQIAGL
AEEGNVVMAWATNTETGFEFQTFGLNRRTPVDLDGLRLVSFLP
3D structure
PDB5dqt Structural and Mechanistic Basis of PAM-Dependent Spacer Acquisition in CRISPR-Cas Systems.
ChainE
Resolution3.1 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.-.-
Interaction with ligand
Site
#
Ligand Ligand
chain
Binding residues on receptor
(original residue number in PDB)
Binding residues on receptor
(residue number reindexed from 1)
Binding affinity
BS01 dna E E9 N10 A28 E9 N10 A28
BS02 dna E R14 R77 R78 R14 R77 R78
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0004519 endonuclease activity
GO:0005515 protein binding
Biological Process
GO:0043571 maintenance of CRISPR repeat elements
GO:0051607 defense response to virus
GO:0099048 CRISPR-cas system

View graph for
Molecular Function

View graph for
Biological Process
External links
PDB RCSB:5dqt, PDBe:5dqt, PDBj:5dqt
PDBsum5dqt
PubMed26478180
UniProtP45956|CAS2_ECOLI CRISPR-associated endoribonuclease Cas2 (Gene Name=ygbF)

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