Structure of PDB 5dbj Chain E

Receptor sequence

>5dbjE (length=437) Species: 220664 (Pseudomonas protegens Pf-5)

SDHDYDVVIIGGGPAGSTMASYLAKAGVKCAVFEKELFEREHVGESLVPA
TTPVLLEIGVMEKIEKANFPKKFGAAWTSADSGPEDKMGFQGLDHDFRSA
EILFNERKQEGVDRDFTFHVDRGKFDRILLEHAGSLGAKVFQGVEIADVE
FLSPGNVIVNAKLGKRSVEIKAKMVVDASGRNVLLGRRLGLREKDPVFNQ
FAIHSWFDNFDRKSATQSPDKVDYIFIHFLPMTNTWVWQIPITETITSVG
VVTQKQNYTNSDLTYEEFFWEAVKTRENLHDALKASEQVRPFKKEADYSY
GMKEVCGDSFVLIGDAARFVDPIFSSGVSVALNSARIASGDIIEAVKNND
FSKSSFTHYEGMIRNGIKNWYEFITLYYRLNILFTAFVQDPRYRLDILQL
LQGDVYSGKRLEVLDKMREIIAAVESDPEHLWHKYLG

3D structure

• PDB: 5dbj Crystal structure of halogenase PltA from the pyoluteorin biosynthetic pathway.
• Chain: E
• Resolution: 2.75 Å

Enzymatic activity

• Enzyme Commision number: 1.14.19.56: 1H-pyrrole-2-carbonyl-[peptidyl-carrier protein] chlorinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	E	G12 G14 P15 A16 F34 E35 K36 R41 H43 V44 G45 S47 R123 E146 I147 S180 G181 N183 W239 E296 D316 F320 P323 S326 G328 V329	G11 G13 P14 A15 F33 E34 K35 R40 H42 V43 G44 S46 R122 E145 I146 S179 G180 N182 W238 E295 D315 F319 P322 S325 G327 V328
BS02	CL	E	F320 S326 S327 G328	F319 S325 S326 G327

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003824 catalytic activity
• GO:0016491 oxidoreductase activity
• GO:0047651 alkylhalidase activity
• GO:0071949 FAD binding

Biological Process

• GO:0017000 antibiotic biosynthetic process

External links

• PDB: RCSB:5dbj, PDBe:5dbj, PDBj:5dbj
• PDBsum: 5dbj
• PubMed: 26416533
• UniProt: Q4KCZ0|PLTA_PSEF5 1H-pyrrole-2-carbonyl-[peptidyl-carrier protein] chlorinase (Gene Name=pltA)