Structure of PDB 4zla Chain E

Receptor sequence
>4zlaE (length=484) Species: 85962 (Helicobacter pylori 26695) [Search protein sequence]
MLKIKLEKTTFENAKAECSLVFIINKDFSHAWVKNKELLETFKYEGEGVF
LDQENKILYAGVKEDDVHLLRESACLAVRTLKKLAFKSVKVGVYTCGAAL
LENLKALFLGLKLGLYEYDTFKSNKKESVLKEAIVALELHKPSLEKSAKE
ALKYAEIMTESLNIVKDLVNTPPMIGTPVYMAEVAQKVAKENHLEIHVHD
EKFLEEKKMNAFLAVNKASLSVNPPRLIHLVYKPKKAKKKIALVGKGLTY
DCGGLSLKPADYMVTMKADKGGGSAVIGLLNALAKLGVEAEVHGIIGATE
NMIGPAAYKPDDILISKEGKSIEVRNTDAEGRLVLADCLSYAQDLNPDVI
VDFATLTGACVVGLGEFTSAIMGHNEELKNLFETSGLESGELLAKLPFNR
HLKKLIESKIADVCNISSSRYGGAITAGLFLNEFIRDEFKDKWLHIDIAG
PAYVEKEWDVNSFGASGAGVRACTAFVEELLKKA
3D structure
PDB4zla Structural basis for substrate specificity of Helicobacter pylori M17 aminopeptidase.
ChainE
Resolution1.9 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) K270 R344
Catalytic site (residue number reindexed from 1) K258 R332
Enzyme Commision number 3.4.11.1: leucyl aminopeptidase.
3.4.11.10: bacterial leucyl aminopeptidase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ZN E K258 D263 D281 E342 K246 D251 D269 E330
BS02 ZN E D263 D340 E342 D251 D328 E330
BS03 NA E A461 G462 Y465 A449 G450 Y453
BS04 BCT E A341 E342 G343 R344 L368 A329 E330 G331 R332 L356
BS05 BES E K258 D263 M278 D281 D340 E342 T367 L368 T369 G370 K246 D251 M266 D269 D328 E330 T355 L356 T357 G358 MOAD: ic50=49.9nM
PDBbind-CN: -logKd/Ki=7.30,IC50=49.9nM
Gene Ontology
Molecular Function
GO:0004177 aminopeptidase activity
GO:0008235 metalloexopeptidase activity
GO:0030145 manganese ion binding
GO:0046872 metal ion binding
GO:0070006 metalloaminopeptidase activity
Biological Process
GO:0006508 proteolysis
GO:0019538 protein metabolic process
Cellular Component
GO:0005737 cytoplasm

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Cellular Component
External links
PDB RCSB:4zla, PDBe:4zla, PDBj:4zla
PDBsum4zla
PubMed26616008
UniProtO25294|AMPA_HELPY Cytosol aminopeptidase (Gene Name=pepA)

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