Structure of PDB 4kpl Chain E

Receptor sequence

>4kplE (length=389) Species: 290398 (Chromohalobacter israelensis DSM 3043)

LKIRDAYTIVTCPGRNFVTLKIVTESGTHGIGDATLNGREMAVAAYLDEH
VVPALIGRDAGRIEDTWQYLYRGAYWRRGPVTMTAIAAVDMALWDIKAKA
AGMPLYQLLGGKSRERVMTYAHCTGQTIEDCLGEVARHVELGYRAVRVQS
GVPGYEPADSSLPAEHVWSTEKYLNHAPKLFAAVRERFGDDLHVLHDVHH
RLTPIEAARLGKAVEPYHLFWLEDCVPAENQESLRLIREHTTTPLAIGEV
FNSIHDCRELIQNQWIDYIRMPLTHGGGITAMRRVADLASLYHVRTGFHG
PTDLSPVCLGAAIHFDTWVPNFGIQEHMPHTDETDAVFPHDYRFEDGHFL
AGESPGHGVDIDEELAAKYPYERASLPVNRLEDGTLWHW

3D structure

• PDB: 4kpl Crystal structure of d-mannonate dehydratase from chromohalobacter salexigens complexed with Mg,d-mannonate and 2-keto-3-deoxy-d-gluconate
• Chain: E
• Resolution: 2.0 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H124 R149 Q151 D213 H215 E239 G264 E265 R286 P288 H315 G316 E342 W405
• Catalytic site (residue number reindexed from 1): H122 R147 Q149 D197 H199 E223 G248 E249 R270 P272 H299 G300 E326 W389
• Enzyme Commision number: 4.2.1.-
  4.2.1.39: gluconate dehydratase.
  4.2.1.8: mannonate dehydratase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CS2	E	R149 D213 H215 E265 H315 P317 D319 E342	R147 D197 H199 E249 H299 P301 D303 E326
BS02	MG	E	D213 E239 E265	D197 E223 E249

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0008927 mannonate dehydratase activity
• GO:0016829 lyase activity
• GO:0046872 metal ion binding
• GO:0047929 gluconate dehydratase activity

Biological Process

• GO:0009063 amino acid catabolic process
• GO:0016052 carbohydrate catabolic process

External links

• PDB: RCSB:4kpl, PDBe:4kpl, PDBj:4kpl
• PDBsum: 4kpl
• UniProt: Q1QT89|DMGD_CHRSD D-galactonate dehydratase family member ManD (Gene Name=manD)