Structure of PDB 4k4s Chain E

Receptor sequence

>4k4sE (length=462) Species: 12081 (Human poliovirus 1 Mahoney)

GEIQWMRPSKEVGYPIINAPSKTKLEPSAFHYVFEGVKEPAVLTKNDPRL
KTDFEEAIFSKYVGNKITEVDEYMKEAVDHYAGQLMSLDINTEQMCLEDA
MYGTDGLEALDLSTSAGYPYVAMGKKKRDILNKQTRDTKEMQKLLDTYGI
NLPLVTYVKDELRSKTKVEQGKSRLIEASSLNDSVAMRMAFGNLYAAFHK
NPGVITGSAVGCDPDLFWSKIPVLMEEKLFAFDYTGYDASLSPAWFEALK
MVLEKIGFGDRVDYIDYLNHSHHLYKNKTYCVKGGMPSGCSGTSIFNSMI
NNLIIRTLLLKTYKGIDLDHLKMIAYGDDVIASYPHEVDASLLAQSGKDY
GLTMTPADKSATFETVTWENVTFLKRFFRADEKYPFLIHPVMPMKEIHES
IRWTKDPRNTQDHVRSLCLLAWHNGEEEYNKFLAKIRSVPIGRALDLPEY
STLYRRWLDSFG

3D structure

• PDB: 4k4s Structures of coxsackievirus, rhinovirus, and poliovirus polymerase elongation complexes solved by engineering RNA mediated crystal contacts.
• Chain: E
• Resolution: 2.4 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.
  3.4.22.28: picornain 3C.
  3.4.22.29: picornain 2A.
  3.6.1.15: nucleoside-triphosphate phosphatase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	E	N18 P20 K24 E108 T114 S115 K127 Y157 K159 I176 E177 A178 R188 H199 G211 C212 D213 S288 G289 C290 S291 L419	N18 P20 K24 E108 T114 S115 K127 Y157 K159 I176 E177 A178 R188 H199 G211 C212 D213 S288 G289 C290 S291 L419
BS02	rna	E	K133 Y326 G327 D328 D329 L374 K375 S400 N409 H413	K133 Y326 G327 D328 D329 L374 K375 S400 N409 H413
BS03	ZN	E	H270 H272 C281	H270 H272 C281

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003968 RNA-dependent RNA polymerase activity

Biological Process

• GO:0006351 DNA-templated transcription
• GO:0039694 viral RNA genome replication

External links

• PDB: RCSB:4k4s, PDBe:4k4s, PDBj:4k4s
• PDBsum: 4k4s
• PubMed: 23667424
• UniProt: P03300|POLG_POL1M Genome polyprotein