Structure of PDB 4k4h Chain E

Receptor sequence

>4k4hE (length=326) Species: 9606 (Homo sapiens)

ATNHNLHITEKLEVLAKAYSVQGDKWRALGYAKAINALKSFHKPVTSYQE
ACSIPGIGKRMAEKIIEILESGHLRKLDHISESVPVLELFSNIWGAGTKT
AQMWYQQGFRSLEDIRSQASLTTQQAIGLKHYSDFLERMPREEATEIEQT
VQKAAQAFNSGLLCVACGSYRRGKATCGDVDVLITHPDGRSHRGIFSRLL
DSLRQEGFLTDDLVSQEGQQQKYLGVCRLPGPGRRHRRLDIIVVPYSEFA
CALLYFTGSAHFNRSMRALAKTKGMSLSEHALSTAVVRNTHGCKVGPGRV
LPTPTEKDVFRLLGLPYREPAERDWL

3D structure

• PDB: 4k4h Structural basis for the binding and incorporation of nucleotide analogs with L-stereochemistry by human DNA polymerase lambda.
• Chain: E
• Resolution: 2.1 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D427 D429 D490
• Catalytic site (residue number reindexed from 1): D179 D181 D240
• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.
  4.2.99.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	E	W274 V462 S463 Q464 G468 R514 R517 K521 L527	W26 V214 S215 Q216 G218 R264 R267 K271 L277
BS02	dna	E	W342 G343 A344 G345 K347 T348 K472 R488 Y505	W94 G95 A96 G97 K99 T100 K222 R238 Y255
BS03	dna	E	Y267 W274 R275 G278 Y279 G304 G306 R308 M309 K312	Y19 W26 R27 G30 Y31 G56 G58 R60 M61 K64
BS04	1RZ	E	R386 G416 S417 R420 G426 D427 D429 Y505 F506 A510 N513	R138 G168 S169 R172 G178 D179 D181 Y255 F256 A260 N263
BS05	CA	E	D427 D429	D179 D181
BS06	CA	E	S339 I341 A344	S91 I93 A96
BS07	CA	E	D427 D429 D490	D179 D181 D240
BS08	CA	E	E396 Q400 C412	E148 Q152 C164

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0034061 DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:4k4h, PDBe:4k4h, PDBj:4k4h
• PDBsum: 4k4h
• PubMed: 25015085
• UniProt: Q9UGP5|DPOLL_HUMAN DNA polymerase lambda (Gene Name=POLL)