Structure of PDB 4fip Chain E

Receptor sequence
>4fipE (length=441) Species: 559292 (Saccharomyces cerevisiae S288C) [Search protein sequence]
MSICPHIQQVFQNEKSKDGVLKTCNAARYILNHSVPKEKFLNTMKCGTCH
EINSGATFMCLQCGFCGCWNHSHFLSHSKQIGHIFGINSNNGLLFCFKCE
DYIGNIDLINDAILAKYWDDVCTKTMVPSMERRDGLSGLINMGNTCFMSS
ILQCLIHNPYFIRHSMSQIHSNNCKVRSPDKCFSCALDKIVHELYGALNR
QTGFIYLLTCAWKINQNLAGYSQQDAHEFWQFIINQIHQSYVLDLPNNKQ
CECIVHTVFEGSLESSIVCPGCQNNSKTTIDPFLDLSLDIKDKKKLYECL
DSFHKKEQLKDFNYHCGECNSTQDAIKQLGIHKLPSVLVLQLKRFEHLLN
GSNRKLDDFIEFPTYLNMKNYCSTKVPDIIYELIGIVSHKGTVNEGHYIA
FCKISGGQWFKFNDSMVSSISQEEVLKEQAYLLFYTIRQVN
3D structure
PDB4fip A Role for Intersubunit Interactions in Maintaining SAGA Deubiquitinating Module Structure and Activity.
ChainE
Resolution2.686 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) N141 C146 H427 N443
Catalytic site (residue number reindexed from 1) N141 C146 H397 N413
Enzyme Commision number 3.4.19.12: ubiquitinyl hydrolase 1.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ZN E H170 C174 C182 C185 H170 C174 C182 C185
BS02 ZN E H250 C271 C273 H276 H238 C251 C253 H256
BS03 ZN E C46 C49 C68 H73 C46 C49 C68 H73
BS04 ZN E C289 C292 C336 C339 C269 C272 C316 C319
BS05 ZN E C60 C63 H77 H83 C60 C63 H77 H83
BS06 ZN E H6 C96 C99 H6 C96 C99
Gene Ontology
Molecular Function
GO:0004843 cysteine-type deubiquitinase activity
GO:0005515 protein binding
GO:0008234 cysteine-type peptidase activity
GO:0008270 zinc ion binding
GO:0046872 metal ion binding
GO:0060090 molecular adaptor activity
Biological Process
GO:0006357 regulation of transcription by RNA polymerase II
GO:0006508 proteolysis
GO:0008380 RNA splicing
GO:0016579 protein deubiquitination
Cellular Component
GO:0000124 SAGA complex
GO:0005634 nucleus
GO:0046695 SLIK (SAGA-like) complex
GO:0071819 DUBm complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:4fip, PDBe:4fip, PDBj:4fip
PDBsum4fip
PubMed22771212
UniProtP50102|UBP8_YEAST Ubiquitin carboxyl-terminal hydrolase 8 (Gene Name=UBP8)

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