Structure of PDB 3t8w Chain E

Receptor sequence

>3t8wE (length=509) Species: 36329 (Plasmodium falciparum 3D7)

SEVPQVVSLDPTSIPIEYNTPIHDIKVQVYDIKGGCNVEEGLTIFLVNNP
KENGPVKISSKVNDKQVSEFLKDENMEKFNVKLGTSKHFYMFNDNKNSVA
VGYVGCGSVADLSEADMKRVVLSLVTMLHDNKLSKLTVVFEINVDKNLFR
FFLETLFYEYMTDERFKSEYIKHLGVYINNADTYKEEVEKARVYYFGTYY
ASQLIAAPSNYCNPVSLSNAAVELAQKLNLEYKILGVKELEELKMGAYLS
VGKGSMYPNKFIHLTYKSKGDVKKKIALVGKGITFDSGGYNLKAAPGSMI
DLMKFDMSGCAAVLGCAYCVGTLKPENVEIHFLSAVCENMVSKNSYRPGD
IITASNGKTIEVGNTDAEGRLTLADALVYAEKLGVDYIVDIATLTGAMLY
SLGTSYAGVFGNNEELINKILQSSKTSNEPVWWLPIINEYRATLNSKYAD
INQISSSVKASSIVASLFLKEFVQNTAWAHIDIAGVSWNFKARKPKGFGV
RLLTEFVLN

3D structure

• PDB: 3t8w Bestatin-based chemical biology strategy reveals distinct roles for malaria M1- and M17-family aminopeptidases
• Chain: E
• Resolution: 2.0 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): K386 R463
• Catalytic site (residue number reindexed from 1): K293 R370
• Enzyme Commision number: 3.4.11.1: leucyl aminopeptidase.
  3.4.13.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	DGZ	E	Y493 K552	Y400 K459	MOAD: Ki=330nM
BS02	CO3	E	A460 G462 R463 L487	A367 G369 R370 L394
BS03	ZN	E	K374 D379 D399 E461	K281 D286 D306 E368
BS04	ZN	E	D379 D459 E461	D286 D366 E368
BS05	DGZ	E	K374 D379 A388 G390 M392 M396 F398 D399 N457 D459 E461 T486 L487 T488 G489 A490 Y493 I547	K281 D286 A295 G297 M299 M303 F305 D306 N364 D366 E368 T393 L394 T395 G396 A397 Y400 I454	MOAD: Ki=330nM

Gene Ontology

Molecular Function

• GO:0030145 manganese ion binding
• GO:0046872 metal ion binding
• GO:0070006 metalloaminopeptidase activity

Biological Process

• GO:0006508 proteolysis
• GO:0019538 protein metabolic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:3t8w, PDBe:3t8w, PDBj:3t8w
• PDBsum: 3t8w
• PubMed: 21844374
• UniProt: Q8IL11|AMPL_PLAF7 Leucine aminopeptidase (Gene Name=LAP)