Structure of PDB 3m30 Chain E

Receptor sequence

>3m30E (length=442) Species: 79929 (Methanothermobacter marburgensis str. Marburg)

AKFEDKVDLYDDRGNLVEEQVPLEALSPLRNPAIKSIVQGIKRTVAVNLE
GIENALKTAKVGGPACKIMGRELDLDIVGNAESIAAAAKEMIQVTEDDDT
NVELLGGGKRALVQVPSARFDVAAEYSAAPLVTATAFVQAIINEFDVSMY
DANMVKAAVLGRYPQSVEYMGANIATMLDIPQKLEGPGYALRNIMVNHVV
AATLKNTLQAAALSTILEQTAMFEMGDAVGAFERMHLLGLAYQGMNADNL
VFDLVKANGKEGTVGSVIADLVERALEDGVIKVEKELTDYKVYGTDDLAM
WNAYAAAGLMAATMVNQGAARAAQGVSSTLLYYNDLIEFETGLPSVDFGK
VEGTAVGFSFFSHSIYGGGGPGIFNGNHIVTRHSKGFAIPCVAAAMALDA
GTQMFSPEATSGLIKEVFSQVDEFREPLKYVVEAAAEIKNEI

3D structure

• PDB: 3m30 Structural insight into methyl-coenzyme M reductase chemistry using coenzyme B analogues.
• Chain: E
• Resolution: 1.45 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): Y367
• Catalytic site (residue number reindexed from 1): Y366
• Enzyme Commision number: 2.8.4.1: coenzyme-B sulfoethylthiotransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	F43	E	S365 I366 Y367	S364 I365 Y366
BS02	TP7	E	F362 Y367 G369 H379	F361 Y366 G368 H378
BS03	COM	E	F361 Y367	F360 Y366
BS04	XP9	E	F361 F362 Y367 G369 H379	F360 F361 Y366 G368 H378

Gene Ontology

Molecular Function

• GO:0016740 transferase activity
• GO:0050524 coenzyme-B sulfoethylthiotransferase activity

Biological Process

• GO:0015948 methanogenesis

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:3m30, PDBe:3m30, PDBj:3m30
• PDBsum: 3m30
• PubMed: 20707311
• UniProt: P11560|MCRB_METTM Methyl-coenzyme M reductase I subunit beta (Gene Name=mcrB)