Structure of PDB 3kqu Chain E

Receptor sequence

>3kquE (length=437) Species: 333284 (Hepatitis C virus (isolate Con1))

SPPAVPQTFQVAHLHAPTGSGKSTKVPAAYAAQGYKVLVLNPSVAATLGF
GAYMSKAHGIDPNIRTGVRTITTGAPITYSTYGKFLADGGCSGGAYDIII
CDECHSTDSTTILGIGTVLDQAETAGARLVVLATATPPGSVTVPHPNIEE
VALSSTGEIPFYGKAIPIETIKGGRHLIFCHSKKKCDELAAKLSGLGLNA
VAYYRGLDVSVIPTSGDVIVVATDALMTGFTGDFDSVIDCNTCVTQTVDF
SLDPTFTIETTTVPQDAVSRSQRRGRTGRGRMGIYRFVTPGERPSGMFDS
SVLCECYDAGCAWYELTPAETSVRLRAYLNTPGLPVCQDHLEFWESVFTG
LTHIDAHFLSQTKQAGDNFPYLVAYQATVCARAQAPPPSWDQMWKCLIRL
KPTLHGPTPLLYRLGAVQNEVTTTHPITKYIMACMSA

3D structure

• PDB: 3kqu Inaugural Article: Three conformational snapshots of the hepatitis C virus NS3 helicase reveal a ratchet translocation mechanism.
• Chain: E
• Resolution: 2.4 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.
  3.4.21.98: hepacivirin.
  3.4.22.-
  3.6.1.15: nucleoside-triphosphate phosphatase.
  3.6.4.13: RNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	E	P230 V232 G255 D296 T298 H369 S370 K371 K372 Y392 R393 T411 A413 V432 Q434 T448 W501 Y502 Q552	P42 V44 G67 D108 T110 H181 S182 K183 K184 Y204 R205 T223 A225 V244 Q246 T260 W313 Y314 Q364
BS02	BEF	E	T206 K210 E291 R464 R467	T18 K22 E103 R276 R279
BS03	ADP	E	G207 G209 K210 S211 T212 Y241 T419 R467 G468	G19 G21 K22 S23 T24 Y53 T231 R279 G280
BS04	MN	E	S211 D290 E291	S23 D102 E103

Gene Ontology

Molecular Function

• GO:0004386 helicase activity
• GO:0005524 ATP binding

External links

• PDB: RCSB:3kqu, PDBe:3kqu, PDBj:3kqu
• PDBsum: 3kqu
• PubMed: 20080715
• UniProt: Q9WMX2|POLG_HCVCO Genome polyprotein