Structure of PDB 3j94 Chain E

Receptor sequence
>3j94E (length=482) Species: 10029 (Cricetulus griseus) [Search protein sequence]
KMGIGGLDKEFSDIFRRAFASRVFPPEIVEQMGCKHVKGILLYGPPGCGK
TLLARQIGKMLNAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQRRL
GANSGLHIIIFDEIHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEA
LLRPGRLEVKMEIGLPDEKGRLQILHIHTARMRGHQLLSADVDIKELAVE
TKNFSGAELEGLVRAAQSTAMNRHIDFLASLENDIKPAFGTEDYASYIMN
GIIKWGDPVTRVLDDGELLVQQTKNSDRTPLVSVLLEGPPHSGKTALAAK
IAEESNFPFIKICSPDKMIGFSETAKCQAMKKIFDDAYKSQLSCVVVDDI
ERLLDYVPIGPRFSNLVLQALLVLLKKAPPQGRKLLIIGTTSRKDVLQEM
EMLNAFSTTIHVPNIATGEQLLEALELLGNFKDKERTTIAQQVKGKKVWI
GIKKLLMLIEMSLQMDPEYRVRKFLALLREEG
3D structure
PDB3j94 Mechanistic insights into the recycling machine of the SNARE complex.
ChainE
Resolution4.2 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) K549 T550 D603 D604 K631 K708
Catalytic site (residue number reindexed from 1) K294 T295 D348 D349 K376 K453
Enzyme Commision number 3.6.4.6: vesicle-fusing ATPase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ATP E N505 G506 I507 P545 H546 G548 K549 T550 A551 L552 I707 K708 L711 N250 G251 I252 P290 H291 G293 K294 T295 A296 L297 I452 K453 L456
BS02 ATP E P262 G263 C264 G265 K266 L268 N374 I406 G438 A439 E442 P46 G47 C48 G49 K50 L52 N142 I174 G206 A207 E210
Gene Ontology
Molecular Function
GO:0000149 SNARE binding
GO:0005524 ATP binding
GO:0016787 hydrolase activity
GO:0016887 ATP hydrolysis activity
GO:0017075 syntaxin-1 binding
GO:0019901 protein kinase binding
GO:0019905 syntaxin binding
GO:0030165 PDZ domain binding
GO:0035255 ionotropic glutamate receptor binding
GO:0042802 identical protein binding
GO:0044877 protein-containing complex binding
GO:0046872 metal ion binding
GO:0140545 ATP-dependent protein disaggregase activity
Biological Process
GO:0001921 positive regulation of receptor recycling
GO:0006813 potassium ion transport
GO:0006886 intracellular protein transport
GO:0006891 intra-Golgi vesicle-mediated transport
GO:0015031 protein transport
GO:0035494 SNARE complex disassembly
GO:0043001 Golgi to plasma membrane protein transport
GO:0045732 positive regulation of protein catabolic process
Cellular Component
GO:0005737 cytoplasm
GO:0005794 Golgi apparatus
GO:0005795 Golgi stack
GO:0005829 cytosol
GO:0005886 plasma membrane
GO:0030496 midbody

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Cellular Component
External links
PDB RCSB:3j94, PDBe:3j94, PDBj:3j94
PDBsum3j94
PubMed25581794
UniProtP18708|NSF_CRIGR Vesicle-fusing ATPase (Gene Name=NSF)

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