Structure of PDB 3h8g Chain E

Receptor sequence
>3h8gE (length=497) Species: 303 (Pseudomonas putida) [Search protein sequence]
MELVVKSVAAASVKTATLVIPVGENRKLGAVAKAVDLASEGAISAVLKRG
DLAGKPGQTLLLQNLQGLKAERVLLVGSGKDEALGDRTWRKLVASVAGVL
KGLNGADAVLALDDVAVNNRDAHYGKYRLLAETLLDGEYVFDRFKSQKVE
PRALKKVTLLADKAGQAEVERAVKHASAIATGMAFTRDLGNLPPNLCHPS
FLAEQAKELGKAHKALKVEVLDEKKIKDLGMGAFYAVGQGSDQPPRLIVL
NYQGGKKADKPFVLVGKGITFDTGGISLKPGAGMDEMKYDMCGAASVFGT
LRAVLELQLPVNLVCLLACAENMPSGGATRPGDIVTTMSGQTVEILNTDA
EGRLVLCDTLTYAERFKPQAVIDIATLTGACIVALGSHTTGLMGNNDDLV
GQLLDAGKRADDRAWQLPLFDEYQEQLDSPFADMGNIGGPKAGTITAGCF
LSRFAKAYNWAHMDIAGTAWISGGKDKGATGRPVPLLTQYLLDRAGA
3D structure
PDB3h8g Crystal structure of the leucine aminopeptidase from Pseudomonas putida reveals the molecular basis for its enantioselectivity and broad substrate specificity.
ChainE
Resolution1.5 Å
3D
structure
Catalytic site residues are labeled in the structure
[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]
Enzymatic activity
Catalytic site (original residue number in PDB) K279 R353
Catalytic site (residue number reindexed from 1) K279 R353
Enzyme Commision number 3.4.11.1: leucyl aminopeptidase.
3.4.11.10: bacterial leucyl aminopeptidase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ZN E K267 D272 D290 E351 K267 D272 D290 E351
BS02 MN E D272 K279 D349 E351 D272 K279 D349 E351
BS03 BCT E A350 E351 G352 R353 L377 A350 E351 G352 R353 L377
BS04 BES E K267 D272 M287 D290 D349 E351 T376 L377 W470 K267 D272 M287 D290 D349 E351 T376 L377 W470
Gene Ontology
Molecular Function
GO:0004177 aminopeptidase activity
GO:0008235 metalloexopeptidase activity
GO:0030145 manganese ion binding
GO:0046872 metal ion binding
GO:0070006 metalloaminopeptidase activity
Biological Process
GO:0006508 proteolysis
GO:0019538 protein metabolic process
Cellular Component
GO:0005737 cytoplasm

View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:3h8g, PDBe:3h8g, PDBj:3h8g
PDBsum3h8g
PubMed20359484
UniProtO86436|AMPA_PSEPU Cytosol aminopeptidase (Gene Name=pepA)

[Back to BioLiP]