Structure of PDB 3h8f Chain E

Receptor sequence

>3h8fE (length=497) Species: 303 (Pseudomonas putida)

MELVVKSVAAASVKTATLVIPVGENRKLGAVAKAVDLASEGAISAVLKRG
DLAGKPGQTLLLQNLQGLKAERVLLVGSGKDEALGDRTWRKLVASVAGVL
KGLNGADAVLALDDVAVNNRDAHYGKYRLLAETLLDGEYVFDRFKSQKVE
PRALKKVTLLADKAGQAEVERAVKHASAIATGMAFTRDLGNLPPNLCHPS
FLAEQAKELGKAHKALKVEVLDEKKIKDLGMGAFYAVGQGSDQPPRLIVL
NYQGGKKADKPFVLVGKGITFDTGGISLKPGAGMDEMKYDMCGAASVFGT
LRAVLELQLPVNLVCLLACAENMPSGGATRPGDIVTTMSGQTVEILNTDA
EGRLVLCDTLTYAERFKPQAVIDIATLTGACIVALGSHTTGLMGNNDDLV
GQLLDAGKRADDRAWQLPLFDEYQEQLDSPFADMGNIGGPKAGTITAGCF
LSRFAKAYNWAHMDIAGTAWISGGKDKGATGRPVPLLTQYLLDRAGA

3D structure

• PDB: 3h8f Crystal structure of the leucine aminopeptidase from Pseudomonas putida reveals the molecular basis for its enantioselectivity and broad substrate specificity.
• Chain: E
• Resolution: 2.2 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): K279 R353
• Catalytic site (residue number reindexed from 1): K279 R353
• Enzyme Commision number: 3.4.11.1: leucyl aminopeptidase.
  3.4.11.10: bacterial leucyl aminopeptidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	E	K267 D272 D290 E351	K267 D272 D290 E351
BS02	MN	E	D272 D349 E351	D272 D349 E351
BS03	BCT	E	A350 G352 R353 L377	A350 G352 R353 L377

Gene Ontology

Molecular Function

• GO:0004177 aminopeptidase activity
• GO:0008235 metalloexopeptidase activity
• GO:0030145 manganese ion binding
• GO:0046872 metal ion binding
• GO:0070006 metalloaminopeptidase activity

Biological Process

• GO:0006508 proteolysis
• GO:0019538 protein metabolic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:3h8f, PDBe:3h8f, PDBj:3h8f
• PDBsum: 3h8f
• PubMed: 20359484
• UniProt: O86436|AMPA_PSEPU Cytosol aminopeptidase (Gene Name=pepA)