Structure of PDB 3glf Chain E

Receptor sequence
>3glfE (length=334) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYALSRYLLC
QQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGVDAVREVTEKL
NEHARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPER
LLATLRSRCRLHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGA
ALALFQGDNWQARETLCQALAYSVPSGDWYSLLAALNHEQAPARLHWLAT
LLMDALKRHHGAAQVTNVDVPGLVAELANHLSPSRLQAILGDVCHIREQL
MSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
3D structure
PDB3glf The mechanism of ATP-dependent primer-template recognition by a clamp loader complex.
ChainE
Resolution3.388 Å
3D
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Enzymatic activity
Enzyme Commision number 2.7.7.7: DNA-directed DNA polymerase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna E G89 V90 R94 T121 G89 V90 R94 T121 PDBbind-CN: Kd=0.18uM
BS02 ZN E C50 C62 C65 C50 C62 C65
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003689 DNA clamp loader activity
GO:0003887 DNA-directed DNA polymerase activity
GO:0005515 protein binding
GO:0008408 3'-5' exonuclease activity
Biological Process
GO:0006260 DNA replication
GO:0006261 DNA-templated DNA replication
GO:0071897 DNA biosynthetic process
Cellular Component
GO:0009360 DNA polymerase III complex
GO:0030894 replisome
GO:0043846 DNA polymerase III, clamp loader complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:3glf, PDBe:3glf, PDBj:3glf
PDBsum3glf
PubMed19450514
UniProtP28631|HOLB_ECOLI DNA polymerase III subunit delta' (Gene Name=holB)

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