Structure of PDB 2gwc Chain E

Receptor sequence
>2gwcE (length=436) Species: 3707 (Brassica juncea) [Search protein sequence]
EPLTREDLIAYLASGCKSKEKWRIGTEHEKFGFEVNTLRPMKYDQIAELL
NSIAERFEWEKVMEGDKIIGLKQGKQSISLEPGGQFELSGAPLETLHQTC
AEVNSHLYQVKAVAEEMGIGFLGMGFQPKWRREDIPTMPKGRYDIMRNYM
PKVGSLGLDMMLRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANS
PFTEGKPNGFLSMRSHIWTDTDKDRTGMLPFVFDDSFGFEQYVDYALDVP
MYFAYRNGKYVDCTGMTFRQFLAGKLPCLPGELPTYNDWENHLTTIFPEV
RLKRYMEMRGADGGPWRRLCALPAFWVGLLYDEDVLQSVLDLTADWTPAE
REMLRNKVPVTGLKTPFRDGLLKHVAEDVLKLAKDGLERRGYKEVGFLNA
VTEVVRTGVTPAENLLEMYNGEWGQSVDPVFQELLY
3D structure
PDB2gwc Structural basis for the redox control of plant glutamate cysteine ligase.
ChainE
Resolution2.18 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 6.3.2.2: glutamate--cysteine ligase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 MG E E107 E159 E165 E29 E81 E87
BS02 BSC E E107 E159 P160 M224 T242 C243 T244 R292 F375 R387 E29 E81 P82 M146 T164 C165 T166 R214 F297 R309
Gene Ontology
Molecular Function
GO:0003824 catalytic activity
GO:0004357 glutamate-cysteine ligase activity
Biological Process
GO:0006750 glutathione biosynthetic process
GO:0042398 cellular modified amino acid biosynthetic process

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Molecular Function
View graph for
Biological Process
External links
PDB RCSB:2gwc, PDBe:2gwc, PDBj:2gwc
PDBsum2gwc
PubMed16766527
UniProtO23736|GSH1_BRAJU Glutamate--cysteine ligase, chloroplastic (Gene Name=GSH1)

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