Structure of PDB 1zpu Chain E

Receptor sequence

>1zpuE (length=529) Species: 4932 (Saccharomyces cerevisiae)

ETHTFNWTTGWDYRNVDGLKSRPVITCNGQFPWPDITVNKGDRVQIYLTN
GMNNTNTSMHFHGLFQNGTASMDGVPFLTQCPIAPGSTMLYNFTVDYNVG
TYWYHSHTDGQYEDGMKGLFIIKDDSFPYDYDEELSLSLSEWYHDLVTDL
TKSFMSVYNPTGAEPIPQNLIVNNTMNLTWEVQPDTTYLLRIVNVGGFVS
QYFWIEDHEMTVVEIDGITTEKNVTDMLYITVAQRYTVLVHTKNDTDKNF
AIMQKFDDTMLDVIPSDLQLNATSYMVYNKTAALPTQNYVDSIDNFLDDF
YLQPYEKEAIYGEPDHVITVDVVMDNLKNGVNYAFFNNITYTAPKVPTLM
TVLSSGDQANNSEIYGSNTHTFILEKDEIVEIVLNNQDTGTHPFHLHGHA
FQTIQRDRTYDDALGEVPHSFDPDNHPAFPEYPMRRDTLYVRPQSNFVIR
FKADNPGVWFFHCHIEWHLLQGLGLVLVEDPFGIQDAHSQQLSENHLEVC
QSCSVATEGNAAANTLDLTDLTGENVQHA

3D structure

• PDB: 1zpu The copper-iron connection in biology: Structure of the metallo-oxidase Fet3p.
• Chain: E
• Resolution: 2.8 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H81 H83 G84 Q87 H126 S127 H128 Q132 M137 H413 H416 H418 H483 C484 H485 I486 H489 L494
• Catalytic site (residue number reindexed from 1): H60 H62 G63 Q66 H105 S106 H107 Q111 M116 H392 H395 H397 H462 C463 H464 I465 H468 L473
• Enzyme Commision number: 1.16.3.1: ferroxidase.
  1.16.3.4: cuproxidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MAN	E	L40 Q189 N190 D288 Q290	L19 Q168 N169 D267 Q269
BS02	CU1	E	H413 C484 H489	H392 C463 H468
BS03	CU1	E	H128 H418 H483	H107 H397 H462
BS04	CU1	E	H83 H126 H485	H62 H105 H464
BS05	CU1	E	H81 H416	H60 H395

Gene Ontology

Molecular Function

• GO:0005507 copper ion binding
• GO:0016491 oxidoreductase activity

Biological Process

• GO:0006811 monoatomic ion transport

External links

• PDB: RCSB:1zpu, PDBe:1zpu, PDBj:1zpu
• PDBsum: 1zpu
• PubMed: 16230618
• UniProt: P38993|FET3_YEAST Iron transport multicopper oxidase FET3 (Gene Name=FET3)