Structure of PDB 1qzf Chain E

Receptor sequence
>1qzfE (length=519) Species: 237895 (Cryptosporidium hominis) [Search protein sequence]
EKNVSIVVAASVLSSGIGINGQLPWSISEDLKFFSKITNNKCDSNKKNAL
IMGRKTWDSIGRRPLKNRIIVVISSSLPQDEADPNVVVFRNLEDSIENLM
NDDSIENIFVCGGESIYRDALKDNFVDRIYLTRVALEDIEFDTYFPEIPE
TFLPVYMSQTFCTKNISYDFMIFEKQEKKTLQNCDPARGQLKSIDDTVDL
LGEIFGIRKMGNRHKFPKEEIYNTPSIRFGREHYEFQYLDLLSRVLENGA
YRENRTGISTYSIFGQMMRFDMRESFPLLTTKKVAIRSIFEELIWFIKGD
TNGNHLIEKKVYIWSGNGSKEYLERIGLGHREENDLGPIYGFQWRHYNGE
YKTMHDDYTGVGVDQLAKLIETLKNNPKDRRHILTAWNPSALSQMALPPC
HVLSQYYVTNDNCLSCNLYQRSCDLGLGSPFNIASYAILTMMLAQVCGYE
PGELAIFIGDAHIYENHLTQLKEQLSRTPRPFPQLKFKRKVENIEDFKWE
DIELIGYYPYPTIKMDMAV
3D structure
PDB1qzf Phylogenetic classification of protozoa based on the structure of the linker domain in the bifunctional enzyme, dihydrofolate reductase-thymidylate synthase
ChainE
Resolution2.8 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) L25 D32 E294 W316 Y342 C402 R423 D426
Catalytic site (residue number reindexed from 1) L23 D30 E292 W314 Y340 C400 R421 D424
Enzyme Commision number 1.5.1.3: dihydrofolate reductase.
2.1.1.45: thymidylate synthase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 UMP E R257 C402 R423 N434 Y466 R255 C400 R421 N432 Y464
BS02 NDP E V10 A11 I19 G23 Q24 L25 G55 R56 K57 T58 I75 S76 S77 R92 G115 S117 I118 T145 V8 A9 I17 G21 Q22 L23 G53 R54 K55 T56 I73 S74 S75 R90 G113 S115 I116 T143
Gene Ontology
Molecular Function
GO:0000166 nucleotide binding
GO:0004146 dihydrofolate reductase activity
GO:0004799 thymidylate synthase activity
GO:0008168 methyltransferase activity
GO:0016491 oxidoreductase activity
GO:0016741 transferase activity, transferring one-carbon groups
Biological Process
GO:0006231 dTMP biosynthetic process
GO:0006730 one-carbon metabolic process
GO:0009165 nucleotide biosynthetic process
GO:0032259 methylation
GO:0046654 tetrahydrofolate biosynthetic process
Cellular Component
GO:0005739 mitochondrion
GO:0005829 cytosol

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:1qzf, PDBe:1qzf, PDBj:1qzf
PDBsum1qzf
PubMed14555647
UniProtQ27552

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