Structure of PDB 1p8j Chain E

Receptor sequence

>1p8jE (length=467) Species: 10090 (Mus musculus)

VYQEPTDPKFPQQWYLSGVTQRDLNVKEAWAQGFTGHGIVVSILDDGIEK
NHPDLAGNYDPGASFDVNDQDPDPQPRYTQMNDNRHGTRCAGEVAAVANN
GVCGVGVAYNARIGGVRMLDGEVTDAVEARSLGLNPNHIHIYSASWGPED
DGKTVDGPARLAEEAFFRGVSQGRGGLGSIFVWASGNGGREHDSCNCDGY
TNSIYTLSISSATQFGNVPWYSEACSSTLATTYSSGNQNEKQIVTTDLRQ
KCTESHTGTSASAPLAAGIIALTLEANKNLTWRDMQHLVVQTSKPAHLNA
DDWATNGVGRKVSHSYGYGLLDAGAMVALAQNWTTVAPQRKCIVEILVEP
KDIGKRLEVRKAVTACLGEPNHITRLEHVQARLTLSYNRRGDLAIHLISP
MGTRSTLLAARPHDYSADGFNDWAFMTTHSWDEDPAGEWVLEIENTSEAN
NYGTLTKFTLVLYGTAP

3D structure

• PDB: 1p8j The Crystal Structure of the Proprotein Processing Proteinase Furin Explains its Stringent Specificity
• Chain: E
• Resolution: 2.6 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D153 H194
• Catalytic site (residue number reindexed from 1): D45 H86
• Enzyme Commision number: 3.4.21.75: furin.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	E	D154 D191 N192 H194 E236 S253 W254 G255 P256 D258 D264 G294 N295 D306 Y308 S368	D46 D83 N84 H86 E128 S145 W146 G147 P148 D150 D156 G186 N187 D198 Y200 S260
BS02	peptide	E	H194 S368	H86 S260
BS03	CA	E	D115 D162 V205 N208 V210 G212	D7 D54 V97 N100 V102 G104
BS04	CA	E	D258 D301 E331	D150 D193 E223

Gene Ontology

Molecular Function

• GO:0004252 serine-type endopeptidase activity
• GO:0008236 serine-type peptidase activity

Biological Process

• GO:0006508 proteolysis

External links

• PDB: RCSB:1p8j, PDBe:1p8j, PDBj:1p8j
• PDBsum: 1p8j
• PubMed: 12794637
• UniProt: P23188|FURIN_MOUSE Furin (Gene Name=Furin)