Structure of PDB 1apm Chain E
Receptor sequence
>1apmE (length=341) Species:
10090
(Mus musculus) [
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SEQESVKEFLAKAKEDFLKKWETPSQNTAQLDQFDRIKTLGTGSFGRVML
VKHKESGNHYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVKLEFS
FKDNSNLYMVMEYVAGGEMFSHLRRIGRFAEPHARFYAAQIVLTFEYLHS
LDLIYRDLKPENLLIDQQGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEI
ILSKGYNKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVRFPS
HFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFATTDWIAIYQRK
VEAPFIPKFKGPGDTSNFDDYEEEEIRVSINEKCGKEFTEF
3D structure
PDB
1apm
2.0 A refined crystal structure of the catalytic subunit of cAMP-dependent protein kinase complexed with a peptide inhibitor and detergent.
Chain
E
Resolution
2.0 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB)
N171 D184
Catalytic site (residue number reindexed from 1)
N162 D175
Enzyme Commision number
2.7.11.11
: cAMP-dependent protein kinase.
Interaction with ligand
Site
#
Ligand
Ligand
chain
Binding residues on receptor
(original residue number in PDB)
Binding residues on receptor
(residue number reindexed from 1)
Binding affinity
BS01
peptide
E
T51 E127 F129 R133 E170 F187 L198 G200 E203 E230 Y235 P236 F239 D241 Y330
T42 E118 F120 R124 E161 F178 L189 G191 E194 E221 Y226 P227 F230 D232 Y321
Gene Ontology
Molecular Function
GO:0000287
magnesium ion binding
GO:0004672
protein kinase activity
GO:0004674
protein serine/threonine kinase activity
GO:0004679
AMP-activated protein kinase activity
GO:0004691
cAMP-dependent protein kinase activity
GO:0004712
protein serine/threonine/tyrosine kinase activity
GO:0005515
protein binding
GO:0005524
ATP binding
GO:0019901
protein kinase binding
GO:0019904
protein domain specific binding
GO:0030145
manganese ion binding
GO:0031625
ubiquitin protein ligase binding
GO:0034237
protein kinase A regulatory subunit binding
GO:0106310
protein serine kinase activity
Biological Process
GO:0000122
negative regulation of transcription by RNA polymerase II
GO:0001707
mesoderm formation
GO:0001843
neural tube closure
GO:0006397
mRNA processing
GO:0006468
protein phosphorylation
GO:0006611
protein export from nucleus
GO:0007189
adenylate cyclase-activating G protein-coupled receptor signaling pathway
GO:0007193
adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway
GO:0016310
phosphorylation
GO:0018105
peptidyl-serine phosphorylation
GO:0032024
positive regulation of insulin secretion
GO:0034605
cellular response to heat
GO:0045542
positive regulation of cholesterol biosynthetic process
GO:0045667
regulation of osteoblast differentiation
GO:0045722
positive regulation of gluconeogenesis
GO:0045879
negative regulation of smoothened signaling pathway
GO:0046827
positive regulation of protein export from nucleus
GO:0048240
sperm capacitation
GO:0050804
modulation of chemical synaptic transmission
GO:0051726
regulation of cell cycle
GO:0061136
regulation of proteasomal protein catabolic process
GO:0070417
cellular response to cold
GO:0070613
regulation of protein processing
GO:0071333
cellular response to glucose stimulus
GO:0071374
cellular response to parathyroid hormone stimulus
GO:0071377
cellular response to glucagon stimulus
GO:0099170
postsynaptic modulation of chemical synaptic transmission
GO:0141156
cAMP/PKA signal transduction
GO:1904262
negative regulation of TORC1 signaling
GO:1904539
negative regulation of glycolytic process through fructose-6-phosphate
GO:1990044
protein localization to lipid droplet
GO:2000810
regulation of bicellular tight junction assembly
Cellular Component
GO:0001669
acrosomal vesicle
GO:0005634
nucleus
GO:0005654
nucleoplasm
GO:0005737
cytoplasm
GO:0005739
mitochondrion
GO:0005813
centrosome
GO:0005829
cytosol
GO:0005886
plasma membrane
GO:0005930
axoneme
GO:0005952
cAMP-dependent protein kinase complex
GO:0016607
nuclear speck
GO:0031410
cytoplasmic vesicle
GO:0031514
motile cilium
GO:0031594
neuromuscular junction
GO:0036126
sperm flagellum
GO:0044853
plasma membrane raft
GO:0048471
perinuclear region of cytoplasm
GO:0097546
ciliary base
GO:0098794
postsynapse
GO:0098978
glutamatergic synapse
View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB
RCSB:1apm
,
PDBe:1apm
,
PDBj:1apm
PDBsum
1apm
PubMed
15299526
UniProt
P05132
|KAPCA_MOUSE cAMP-dependent protein kinase catalytic subunit alpha (Gene Name=Prkaca)
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