Structure of PDB 4wqy Chain DZ

Receptor sequence

>4wqyDZ (length=730) Species: 300852 (Thermus thermophilus HB8)

MKVILLEPLENLGDVGQVVDVKPGYARNYLLPRGLAVLATESNLKALEAR
IRAQAKRLAERKAEAERLKEILENDLKRLRNIGIAAHIDAGKTTTTERIL
YYTGRIHAAVTTCFWKDHRINIIDTPGHVDFTIEVERSMRVLDGAIVVFD
SSQGVEPQSETVWRQAEKYKVPRIAFANKMDKTGADLWLVIRTMQERLGA
RPVVMQLPIGREDTFSGIIDVLRMKAYTYGNDLGTDIREIPIPEEYLDQA
REYHEKLVEVAADFDENIMLKYLEGEEPTEEELVAAIRKGTIDLKITPVF
LGSALKNKGVQLLLDAVVDYLPSPLDIPPIKGTTPEGEVVEIHPDPNGPL
AALAFKIMADPYVGRLTFIRVYSGTLTSGSYVYNTTKGRKERVARLLRMH
ANHREEVEELKAGDLGAVVGLKETITGDTLVGEDAPRVILESIEVPEPVI
DVAIEPKTKADQEKLSQALARLAEEDPTFRVSTHPETGQTIISGMGELHL
EIIVDRLKREFKVDANVGKPQVAYRETITKPVDVEGKFIRQTGGRGQYGH
VKIKVEPLPRGSGFEFVNAIVGGVIPKEYIPAVQKGIEEAMQSGPLIGFP
VVDIKVTLYDGSYHEVDSSEMAFKIAGSMAIKEAVQKGDPVILEPIMRVE
VTTPEEYMGDVIGDLNARRGQILGMEPRGNAQVIRAFVPLAEMFGYATDL
RSKTQGRGSFVMFFDHYQEVPKQVQEKLIK

3D structure

• PDB: 4wqy Conformational Changes of Elongation Factor G on the Ribosome during tRNA Translocation.
• Chain: DZ
• Resolution: 2.8 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	DZ	M617 G618 I621 I631 L632 G633 S661	M658 G659 I662 I672 L673 G674 S702
BS02	rna	DZ	G323 R324 Y340 K349 R351 A353 R354 K381 V533	G364 R365 Y381 K390 R392 A394 R395 K422 V574
BS03	rna	DZ	G502 G503 R504	G543 G544 R545
BS04	rna	DZ	Q500 T501 G502	Q541 T542 G543
BS05	GDP	DZ	D22 G24 K25 T26 T27 N137 K138 D140 S262 A263 L264	D89 G91 K92 T93 T94 N178 K179 D181 S303 A304 L305

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0003746 translation elongation factor activity
• GO:0003924 GTPase activity
• GO:0005525 GTP binding

Biological Process

• GO:0006412 translation
• GO:0006414 translational elongation
• GO:0032790 ribosome disassembly

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome

External links

• PDB: RCSB:4wqy, PDBe:4wqy, PDBj:4wqy
• PDBsum: 4wqy
• PubMed: 25594181
• UniProt: Q5SHN5|EFG_THET8 Elongation factor G (Gene Name=fusA);
  Q5SLQ1|RL9_THET8 Large ribosomal subunit protein bL9 (Gene Name=rplI)