Structure of PDB 5it8 Chain DP

Receptor sequence

>5it8DP (length=117) Species: 83333 (Escherichia coli K-12)

MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNGSEVLVA
ASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYH
GRVQALADAAREAGLQF

3D structure

• PDB: 5it8 Resistance mutations generate divergent antibiotic susceptibility profiles against translation inhibitors.
• Chain: DP
• Resolution: 3.12 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	DP	K3 K4 R15 R25 R30 T31 P32 R33 H34 Y36 Q38 I40 G44 S45 E46 V47 V54 K63 Y64 N67 K68 Q98 H100 G101 R102	K3 K4 R15 R25 R30 T31 P32 R33 H34 Y36 Q38 I40 G44 S45 E46 V47 V54 K63 Y64 N67 K68 Q98 H100 G101 R102
BS02	rna	DP	R9 R10 T12 R13 R16 K17 L18 L21 S91 R94 Q98 Y99 R111 F117	R9 R10 T12 R13 R16 K17 L18 L21 S91 R94 Q98 Y99 R111 F117

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0008097 5S rRNA binding
• GO:0019843 rRNA binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:5it8, PDBe:5it8, PDBj:5it8
• PDBsum: 5it8
• PubMed: 27382179
• UniProt: P0C018|RL18_ECOLI Large ribosomal subunit protein uL18 (Gene Name=rplR)