Structure of PDB 5j8a Chain DL

Receptor sequence

>5j8aDL (length=123) Species: 83333 (Escherichia coli K-12)

MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIKEAIPRG
KVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIF
GPVTRELRSEKFMKIISLAPEVL

3D structure

• PDB: 5j8a Resistance mutations generate divergent antibiotic susceptibility profiles against translation inhibitors.
• Chain: DL
• Resolution: 3.1 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	DL	N13 P48 R49 T97 R98 R105 R108 S117	N13 P48 R49 T97 R98 R105 R108 S117
BS02	rna	DL	M1 Q3 E4 Q5 T6 M7 I22 K23 L25 G26 G27 S28 H29 R30 R31 Y32 K40 T42 K44 K54 V57 K66 K67 R70 V76 R78 N82	M1 Q3 E4 Q5 T6 M7 I22 K23 L25 G26 G27 S28 H29 R30 R31 Y32 K40 T42 K44 K54 V57 K66 K67 R70 V76 R78 N82

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0019843 rRNA binding
• GO:0070180 large ribosomal subunit rRNA binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:5j8a, PDBe:5j8a, PDBj:5j8a
• PDBsum: 5j8a
• PubMed: 27382179
• UniProt: P0ADY3|RL14_ECOLI Large ribosomal subunit protein uL14 (Gene Name=rplN)