Structure of PDB 5j8a Chain DF

Receptor sequence

>5j8aDF (length=177) Species: 83333 (Escherichia coli K-12) [Search protein sequence]

AKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAIADKKLLD
NAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFE
RLITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRG
LDITITTTAKSDEEGRALLAAFDFPFR

3D structure

PDB

5j8a Resistance mutations generate divergent antibiotic susceptibility profiles against translation inhibitors.

Chain

Resolution

3.1 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	DF	S24 M26 Q27 Q63 K64 L66 K69 V89 T90 R92	S23 M25 Q26 Q62 K63 L65 K68 V88 T89 R91
BS02	rna	DF	K33 N37 M38 G39 V40 G41 T68 A70 R71 K72 F77 I85 K88 S121 D123 R125 N127 S129 M130 G131 V132 R133 G151 L152 D153 T155 T157	K32 N36 M37 G38 V39 G40 T67 A69 R70 K71 F76 I84 K87 S120 D122 R124 N126 S128 M129 G130 V131 R132 G150 L151 D152 T154 T156

Gene Ontology

	Molecular Function
GO:0000049	tRNA binding
GO:0003723	RNA binding
GO:0003735	structural constituent of ribosome
GO:0008097	5S rRNA binding
GO:0019843	rRNA binding

	Biological Process
GO:0000027	ribosomal large subunit assembly
GO:0002181	cytoplasmic translation
GO:0006412	translation

	Cellular Component
GO:0005737	cytoplasm
GO:0005829	cytosol
GO:0005840	ribosome
GO:0022625	cytosolic large ribosomal subunit
GO:1990904	ribonucleoprotein complex

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:5j8a, PDBe:5j8a, PDBj:5j8a
PDBsum	5j8a
PubMed	27382179
UniProt	P62399\|RL5_ECOLI Large ribosomal subunit protein uL5 (Gene Name=rplE)

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