Structure of PDB 7orx Chain DDD

Receptor sequence

>7orxDDD (length=527) Species: 101510 (Rhodococcus jostii RHA1) [Search protein sequence]

ATVSEVTYELLRARGLTTVFGNPGSNELPFLSGMPDDFRYVLGLHEGAVL
SMADGYSLVTGEATLVNLHAASGSGNAMGALTNSVYSHSPLVVTAGQQVR
STIGQEVMLSNVDAGTLMKPLVKWSSEPTCAEDVPRTINQAIHTALLPAK
GPVYVSVPYDDWAAEAPPESAGLLAREVHSAASLSGDQINDLIETLESAT
NPVLVLGPAVDADRANADAVLLAEKLRAPVWIAPSPSRCPFPTRHPSFRG
VLPAGVADLSKTLEGHDLILVVGAPVFRYHQYVPGNYLPGGARLIHVTDD
GGEAARAPIGEAYVAPVGSTLEILANMVKPSDRSPLPPLGDFEEAVSVGA
GLDPAQLFALVRAGAPDDAIYVNESTSTSDAFWSQMDLSHQGSYYFPASG
GLGFGLPAAVGAQLASPDRQVIGLIGDGSANYGITALWSAAQYKIPVVII
ILNNGTYGALRGFSKILNTGETPGLDVPGIDFVHLAEGYGVRGTAVATAE
DFTTAFKSALAADAPTLIEVRTNFDES

3D structure

PDB

7orx Characterization of Thiamine Diphosphate-Dependent 4-Hydroxybenzoylformate Decarboxylase Enzymes from

Chain

DDD

Resolution

2.6 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	N23 G25 S26 N27 E28 E47 H70 M109 L110 S111 N112 Y160 P254 H281 S376 G401 L403 D428 N455 T457 Y458 A460 L461 F464
Catalytic site (residue number reindexed from 1)	N22 G24 S25 N26 E27 E46 H69 M108 L109 S110 N111 Y159 P253 H280 S375 G400 L402 D427 N454 T456 Y457 A459 L460 F463
Enzyme Commision number	2.2.1.6: acetolactate synthase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	TPP	DDD	N23 P24 S26 E47 H70	N22 P23 S25 E46 H69
BS02	TPP	DDD	T377 S378 G401 L403 G427 D428 G429 S430 Y433 N455 T457 Y458 G459 A460	T376 S377 G400 L402 G426 D427 G428 S429 Y432 N454 T456 Y457 G458 A459

Gene Ontology

	Molecular Function
GO:0000287	magnesium ion binding
GO:0003824	catalytic activity
GO:0003984	acetolactate synthase activity
GO:0016829	lyase activity
GO:0030976	thiamine pyrophosphate binding
GO:0050660	flavin adenine dinucleotide binding
GO:0050695	benzoylformate decarboxylase activity

	Biological Process
GO:0009082	branched-chain amino acid biosynthetic process
GO:0009097	isoleucine biosynthetic process
GO:0009099	L-valine biosynthetic process

View graph for
Molecular Function

View graph for
Biological Process

External links

PDB	RCSB:7orx, PDBe:7orx, PDBj:7orx
PDBsum	7orx
PubMed
UniProt	Q0SCE8

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