Structure of PDB 9fea Chain D

Receptor sequence

>9feaD (length=419) Species: 224324 (Aquifex aeolicus VF5)

RSYPAIPRIYAETTLNMLLKRAKKPRVHSIDEYLKDGGYQALEKALNMSP
EEIIDWVDKSTLRGRGGAGFPTGKKWKFAVQNPGPRYFICNADESEPGTF
KDRIIIERDPHLLIEGIIISSYAIGANEAYIYIRGEYPAGYYILRDAIEE
AKKKGFLGKNILGSGFDLEIYVARGAGAYICGEETALIESLEGKRGHPRL
KPPYPVQKGLWGKPTVVNNVETIANVRFIISMGWEEYRYIGPSDYAGPKL
FPVSGKVKKPGVYELPMNTTLREVIFKYAGGTLGNKKVKAVFSGALDCFS
SEELDIPMDYSPLGFGGTGTVIVLTEEDDIVEAALKIAEFYEHETCGQCT
PCRVGCYEQANLLEKIYKGEATEQDWEGFDFVNRNIQPTSICGLGAVAGR
LIRQTLEKFPEEWEKYRKK

3D structure

• PDB: 9fea Structural robustness of the NADH binding site in NADH:ubiquinone oxidoreductase (complex I).
• Chain: D
• Resolution: 1.66 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SF4	D	P199 T346 C347 G348 Q349 C350 C353 I392 C393 L395 G396	P198 T345 C346 G347 Q348 C349 C352 I391 C392 L394 G395
BS02	FNR	D	G65 G67 K76 N92 D94 E95 Y180 G183 E184 V218 N219 N220 T223	G64 G66 K75 N91 D93 E94 Y179 G182 E183 V217 N218 N219 T222
BS03	NAD	D	G68 A69 F71 K76 E185 K202 Y205	G67 A68 F70 K75 E184 K201 Y204

Gene Ontology

Molecular Function

• GO:0046872 metal ion binding
• GO:0048038 quinone binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:1902600 proton transmembrane transport

External links

• PDB: RCSB:9fea, PDBe:9fea, PDBj:9fea
• PDBsum: 9fea
• PubMed: 38960077
• UniProt: O66841|NUOF_AQUAE NADH-quinone oxidoreductase subunit F (Gene Name=nuoF)