Structure of PDB 8wwu Chain D

Receptor sequence

>8wwuD (length=492) Species: 1615674 (Pseudomonas lactis)

KFDFITKNNLWTNEQRDAADKVLAEIDSLGLEMIRLSWADQYGLLRGKSL
TVASLKSAFKEGSEVAIGPFFFDLVSSMVFNLFTTAGDFEDELSGNPTVV
MVPDPTTFKVLPWADKTGWMLADLHWKSGEPFPLCPRGIMKKAVKSLSDE
GYLFKCGIELEWYLTKIVDRSLSPESLGAPGVQPDAIQVQPVAQGYSVLL
EHHLDQVDDIMSKVRKGLLELNLPLRSIEDEWAPSQMETTFDVMEGLEAA
DAALLIKSAIKQICSRHGYHATFMCKPAINGFFASGWHMHQSLVDKDTRK
NLFIPSEGEVLSPLGRAYAGGLLANGSAASSFTTPTVNGYRRRQPYSLAP
DRRAWAKDNKAAMVRVVSATGDPASRIENRIGEPGANPYLYMASQIVSGL
DGIKNKKDPGELQESPYDAQVPMLPTTLAEALDALEHDSELFRSCFGDTF
IKYWLQLRRSEWARFLDAEGAEAAEPTGAVTQWEQKEYFNLL

3D structure

• PDB: 8wwu Discovery of the 1-naphthylamine biodegradation pathway reveals a glutamine synthetase-like protein that catalyzes 1-naphthylamine glutamylation
• Chain: D
• Resolution: 2.0 Å

Enzymatic activity

• Enzyme Commision number: 6.3.1.2: glutamine synthetase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ANP	D	G160 E162 R229 F244 V246 H291 H293 S295 K363 R368 A377 R379 E381	G157 E159 R226 F241 V243 H288 H290 S292 K360 R365 A374 R376 E378
BS02	MN	D	E164 E234 E241	E161 E231 E238
BS03	MN	D	E162 H291 E381	E159 H288 E378
BS04	MG	D	E162 E241	E159 E238

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003824 catalytic activity
• GO:0004356 glutamine synthetase activity
• GO:0016879 ligase activity, forming carbon-nitrogen bonds
• GO:0046872 metal ion binding

Biological Process

• GO:0006542 glutamine biosynthetic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0016020 membrane

External links

• PDB: RCSB:8wwu, PDBe:8wwu, PDBj:8wwu
• PDBsum: 8wwu
• UniProt: A0A7Y1Q2L1