Structure of PDB 8uy2 Chain D

Receptor sequence

>8uy2D (length=557) Species: 759272 (Thermochaetoides thermophila DSM 1495)

SNAMHIRDMLAEAERTGEPSFSFEYFPPKTAQGVQNLYDRMERMYNYGPK
FIDITWGAGGRVAELTCEMVVQAQAYLGLETCMHLTCTDMGVERINDALR
KAYKAGCTNILALRGDPPRDKEKWEAAKDGFRYAKDLVAHIRKEYGDHFD
IGVAGYPEGCDDNKDEDLLLDHLKEKVDMGAGFIVTQMFYDVDNFLRWVK
KVRERGISVPIVPGIMPIATYASFLRRANHMKCKIPEEWMAKLEPVKNDD
VAVREIGKTLVADMCRKILDAGIRHLHFYTMNLAQATRMVLEELNWLPTQ
DWDEFPNGRWGDSRSPAFGELDAYGVGLTGSNEQNRERWGEPKCIRDIAN
LFIRYLRKEIDYLPWSEAPVADEADLIKDELIDLNRRGLITVNSQPAVNG
AKSNHPVHGWGPSNGYVYQKAYLEFFVSPELYPEIKRRIESHPDLTYHAV
TKSGNLETNAQSDGPNAVTWGVFPGKEIVQPTIVERISFLAWKDEAYHLG
MEWARCYDAGSPSRVLLEEMMNTWWLVNIVNNDFHQGNTLFEILKGLEVT
DLDKVPE

3D structure

• PDB: 8uy2 Structural basis of S-adenosylmethionine-dependent allosteric transition from active to inactive states in methylenetetrahydrofolate reductase.
• Chain: D
• Resolution: 2.83 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	D	T52 W53 H81 T83 L110 R111 G112 D113 Y130 A131 A151 Y153 D158 H169 K173 V182 Q184 Y361	T55 W56 H84 T86 L113 R114 G115 D116 Y133 A134 A154 Y156 D161 H172 K176 V185 Q187 Y324
BS02	SAM	D	N245 D247 F342 P343 N344 G446 V516	N248 D250 F305 P306 N307 G409 V479
BS03	SAM	D	N344 Y392 V407 A408 E410 T428 V429 N430 S431 Q432	N307 Y355 V370 A371 E373 T391 V392 N393 S394 Q395

Gene Ontology

Molecular Function

• GO:0004489 methylenetetrahydrofolate reductase (NAD(P)H) activity
• GO:0016491 oxidoreductase activity
• GO:0071949 FAD binding

Biological Process

• GO:0009086 methionine biosynthetic process
• GO:0035999 tetrahydrofolate interconversion

Cellular Component

• GO:0005829 cytosol

External links

• PDB: RCSB:8uy2, PDBe:8uy2, PDBj:8uy2
• PDBsum: 8uy2
• PubMed: 38886362
• UniProt: G0S5U9