Structure of PDB 8tbt Chain D

Receptor sequence

>8tbtD (length=512) Species: 9606 (Homo sapiens) [Search protein sequence]

QQQLPAAMADTFLEHLCLLDIDSEPVAARSTSIIATIGPASRSVERLKEM
IKAGMNIARLNFSHGSHEYHAESIANVREAVESFAGSPLSYRPVAIALDT
KGPEIRTGIESEVELVKGSQVLVTVDPAFRTRGNANTVWVDYPNIVRVVP
VGGRIYIDDGLISLVVQKIGPEGLVTQVENGGVLGSRKGVNLPGAQVDLP
GLSEQDVRDLRFGVEHGVDIVFASFVRKASDVAAVRAALGPEGHGIKIIS
KIENHEGVKRFDEILEVSDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNL
AGKPVVCATQMLESMITKPRPTRAETSDVANAVLDGADCIMLSGETAKGN
FPVEAVKMQHAIAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEA
AFKCCAAAIIVLTTTGRSAQLLSRYRPRAAVIAVTRSAQAARQVHLCRGV
FPLLYREPPEAIWADDVDRRVQFGIESGKLRGFLRVGDLVIVVTGWRPGS
GYTNIMRVLSIS

3D structure

PDB

8tbt Structure-Based Design of AG-946, a Pyruvate Kinase Activator.

Chain

Resolution

2.34 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

2.7.1.40: pyruvate kinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	I07	D	F69 Q436 L437 E440	F12 Q374 L375 E378
BS02	FBP	D	L474 T475 T476 T477 S480 W525 R532 G557 R559 P560 G561 S562 Y564 T565	L412 T413 T414 T415 S418 W463 R470 G495 R497 P498 G499 S500 Y502 T503
BS03	PYR	D	K313 E315 A336 G338 D339 T371	K251 E253 A274 G276 D277 T309
BS04	MN	D	E315 D339	E253 D277

Gene Ontology

	Molecular Function
GO:0000287	magnesium ion binding
GO:0003824	catalytic activity
GO:0004743	pyruvate kinase activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0016301	kinase activity
GO:0030955	potassium ion binding
GO:0046872	metal ion binding
GO:0048029	monosaccharide binding

	Biological Process
GO:0001666	response to hypoxia
GO:0006096	glycolytic process
GO:0007584	response to nutrient
GO:0009749	response to glucose
GO:0010038	response to metal ion
GO:0016310	phosphorylation
GO:0032869	cellular response to insulin stimulus
GO:0033198	response to ATP
GO:0042866	pyruvate biosynthetic process
GO:0051591	response to cAMP
GO:0071872	cellular response to epinephrine stimulus

	Cellular Component
GO:0005737	cytoplasm
GO:0005829	cytosol
GO:0070062	extracellular exosome

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:8tbt, PDBe:8tbt, PDBj:8tbt
PDBsum	8tbt
PubMed	38109501
UniProt	P30613\|KPYR_HUMAN Pyruvate kinase PKLR (Gene Name=PKLR)

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