Structure of PDB 8tbs Chain D

Receptor sequence

>8tbsD (length=483) Species: 9606 (Homo sapiens) [Search protein sequence]

QQQQLPAAMADTFLEHLCLLDIDSEPVAARSTSIIATIGPASRSVERLKE
MIKAGMNIARLNFSHGSHEYHAESIANVREAVESFAGSPLSYRPVAIALD
TKGPEIRTGILANTVWVDYPNIVRVVPVGRIYIDDGLISLVVQKIGPEGL
VTQVENGGVLGSRKGVNLPGAPGLSEQDVRDLRFGVEHGVDIVFASFVRK
ASDVAAVRAALGPEGHGIKIISKIENHEGVKRFDEILEVSDGIMVARGDL
GIEIPAEKVFLAQKMMIGRCNLAGKPVVCATQMLESMITKPRPTRAETSD
VANAVLDGADCIMLSGETAKGNFPVEAVKMQHAIAREAEAAVYHRQLFEE
LRRAAPLSRDPTEVTAIGAVEAAFKCCAAAIIVLTTTGRSAQLLSRYRPR
AAVIAVTRSAQAARQVHLCRGVFPLLYREPPEAIWADDVDRRVQFGIESG
KLRGFLRVGDLVIVVTGWRPGSGYTNIMRVLSI

3D structure

PDB

8tbs Structure-Based Design of AG-946, a Pyruvate Kinase Activator.

Chain

Resolution

2.35 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

2.7.1.40: pyruvate kinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	HVI	D	F69 D397 Q436 L437 E440	F13 D307 Q346 L347 E350
BS02	FBP	D	L474 T475 T476 T477 S480 W525 R532 G557 R559 P560 G561 S562 G563 Y564 T565	L384 T385 T386 T387 S390 W435 R442 G467 R469 P470 G471 S472 G473 Y474 T475
BS03	MN	D	E315 D339	E225 D249
BS04	PYR	D	A336 G338 D339 T371	A246 G248 D249 T281

Gene Ontology

	Molecular Function
GO:0000287	magnesium ion binding
GO:0003824	catalytic activity
GO:0004743	pyruvate kinase activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0016301	kinase activity
GO:0030955	potassium ion binding
GO:0046872	metal ion binding
GO:0048029	monosaccharide binding

	Biological Process
GO:0001666	response to hypoxia
GO:0006096	glycolytic process
GO:0007584	response to nutrient
GO:0009749	response to glucose
GO:0010038	response to metal ion
GO:0016310	phosphorylation
GO:0032869	cellular response to insulin stimulus
GO:0033198	response to ATP
GO:0042866	pyruvate biosynthetic process
GO:0051591	response to cAMP
GO:0071872	cellular response to epinephrine stimulus

	Cellular Component
GO:0005737	cytoplasm
GO:0005829	cytosol
GO:0070062	extracellular exosome

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:8tbs, PDBe:8tbs, PDBj:8tbs
PDBsum	8tbs
PubMed	38109501
UniProt	P30613\|KPYR_HUMAN Pyruvate kinase PKLR (Gene Name=PKLR)

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