Structure of PDB 8t2t Chain D

Receptor sequence

>8t2tD (length=387) Species: 39491 (Agathobacter rectalis)

NLMEQILSSDNLNRAYLQVVRNKGAEGVDGMKYTELKEHLAKNGETIKGQ
LRTRKYKPQPGVPTVTDRFIQQAIAQVLTPIYEEQFHDHSYGFRPNRCAQ
QAILTALNIMNDGNDWIVDIDLEKFFDTVNHDKLMTLIGRTIKDGDVISI
VRKYLVSGGNLSPLLANIMLNELDKEMEKRGLNFVRYADDCIIMVGSEMS
ANRVMRNISRFIEEKLGLKVNMTKSKVDRPSGLKYLGFGFYFDPRAHQFK
AKPHAKSVAKFKKRMKELTCRSWGVSNSYKVEKLNQLIRGWINYFKIGSM
KTLCKELDSRIRYRLRMCIWKQWKTPQNQEKNLVKLGIDRNTARRVAYTG
KRIAYVCNKGAVNVAISNKRLASFGLISMLDYYIEKC

3D structure

• PDB: 8t2t Structural insights into intron catalysis and dynamics during splicing
• Chain: D
• Resolution: 3.0 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.49: RNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	D	S309 K361 N378 R381 R382 V383 Y385 T386 K388 K396	S272 K324 N341 R344 R345 V346 Y348 T349 K351 K359
BS02	rna	D	D152 T156 G165 S169 R172 R217 S234 M236 S237 R240 R243 N244 R247 K300 R308 S309 R347 R351 K358 R389 A391 Y392 N395	D132 T136 G145 S149 R152 R180 S197 M199 S200 R203 R206 N207 R210 K263 R271 S272 R310 R314 K321 R352 A354 Y355 N358

Gene Ontology

Molecular Function

• GO:0003964 RNA-directed DNA polymerase activity

Biological Process

• GO:0006278 RNA-templated DNA biosynthetic process
• GO:0006974 DNA damage response

External links

• PDB: RCSB:8t2t, PDBe:8t2t, PDBj:8t2t
• PDBsum: 8t2t
• PubMed: 37993708
• UniProt: A0A173ZME3